| Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris | |
Li, Rongfeng1; Yu, Huahua1 ; Xing, Ronge1; Liu, Song1; Qing, Yukun1; Li, Kecheng1,2; Li, Bing1,2; Meng, Xiangtao1,2; Cui, Jinhui1,2; Li, Pengcheng1; Yu, HH
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| 2013-09-01 | |
| 发表期刊 | TOXICOLOGY IN VITRO
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| ISSN | 0887-2333 |
| 卷号 | 27期号:6页码:1620-1625 |
| 文章类型 | Article |
| 摘要 | Jellyfish venom contains various toxins and can cause itching, edema, muscle aches, shortness of breath, blood pressure depression, shock or even death after being stung. Hemolytic protein is one of the most hazardous components in the venom. The present study investigated the hemolytic activity of the nematocyst venom from jellyfish Stomolophus meleagris. Anion exchange chromatography, DEAE Sepharose Fast Flow, and gel filtration chromatography, Superdex200 had been employed to isolate hemolytic proteins from the nematocyst venom of jellyfish S. meleagris. Hemolysis of chicken red blood cells was used to quantify hemolytic potency of crude nematocyst venom and chromatography fractions during the purification process. Native-PAGE profile displayed one protein band in the purified hemolytic protein (SmTX); however, two protein bands with apparent molecular weights of similar to 45 kDa and 52 kDa were observed in the reducing SDS-PAGE analysis. Approximately 70 mu g/mL of SmTX caused 50% hemolysis (HU50) of the erythrocyte suspension. The hemolytic activity of SmTX was shown to be temperature and pH dependent, with the optimum temperature and pH being 37 degrees C and pH 5.0. The present study is the first report of isolation and partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish S. meleagris. The mechanism of the hemolytic activity of SmTX is not clear and deserves further investigation. (C) 2013 Published by Elsevier Ltd.; Jellyfish venom contains various toxins and can cause itching, edema, muscle aches, shortness of breath, blood pressure depression, shock or even death after being stung. Hemolytic protein is one of the most hazardous components in the venom. The present study investigated the hemolytic activity of the nematocyst venom from jellyfish Stomolophus meleagris. Anion exchange chromatography, DEAE Sepharose Fast Flow, and gel filtration chromatography, Superdex200 had been employed to isolate hemolytic proteins from the nematocyst venom of jellyfish S. meleagris. Hemolysis of chicken red blood cells was used to quantify hemolytic potency of crude nematocyst venom and chromatography fractions during the purification process. Native-PAGE profile displayed one protein band in the purified hemolytic protein (SmTX); however, two protein bands with apparent molecular weights of similar to 45 kDa and 52 kDa were observed in the reducing SDS-PAGE analysis. Approximately 70 mu g/mL of SmTX caused 50% hemolysis (HU50) of the erythrocyte suspension. The hemolytic activity of SmTX was shown to be temperature and pH dependent, with the optimum temperature and pH being 37 degrees C and pH 5.0. The present study is the first report of isolation and partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish S. meleagris. The mechanism of the hemolytic activity of SmTX is not clear and deserves further investigation. (C) 2013 Published by Elsevier Ltd. |
| 关键词 | Jellyfish Stomolophus Meleagris Nematocyst Venom Isolation Hemolysis Smtx |
| 学科领域 | Toxicology |
| DOI | 10.1016/j.tiv.2013.04.004 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Toxicology |
| WOS类目 | Toxicology |
| WOS记录号 | WOS:000324847800003 |
| WOS关键词 | RHOPILEMA-ESCULENTUM KISHINOUYE ; CYANEA-NOZAKII KISHINOUYE ; BOX JELLYFISH ; PARTIAL-PURIFICATION ; SEA WASP ; CARYBDEA-MARSUPIALIS ; CHIRONEX-FLECKERI ; TOXIN ; BINDING ; ALATA |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16706 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 通讯作者 | Yu, HH |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Rongfeng,Yu, Huahua,Xing, Ronge,et al. Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris[J]. TOXICOLOGY IN VITRO,2013,27(6):1620-1625. |
| APA | Li, Rongfeng.,Yu, Huahua.,Xing, Ronge.,Liu, Song.,Qing, Yukun.,...&Yu, HH.(2013).Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris.TOXICOLOGY IN VITRO,27(6),1620-1625. |
| MLA | Li, Rongfeng,et al."Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris".TOXICOLOGY IN VITRO 27.6(2013):1620-1625. |
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