Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis | |
Wang, Di1,3; Zhang, Shujing1; Liu, Baozhong1,2,3
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| 2020-03-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 98页码:1017-1023 |
| 通讯作者 | Liu, Baozhong([email protected]) |
| 摘要 | TAF5L is a component of the P300/CBP-associated factor (PCAF) histone acetylase complex, which serves as a coactivator and takes part in basal transcription such as promoter recognition, complex assembly and transcription initiation. In our study, the full-length sequence of MpTAF5L was identified and characterized in the clam M. petechialis. Sequence analysis showed that the predicted MpTAF5L protein had a N-terminal TAF5-NTD2 domain and a C-terminal WD40-repeats domain. The annotation and evolutionary analysis revealed MpTAF5L had close evolutionary relationship with other invertebrate species. Tissue distribution analysis of TAFSL claimed that it was highly expressed in the mantle, adductor muscle, foot and hepatopancreas. The mRNA expression of MpTAF5L was significantly up-regulated after Vibrio parahaemolyticus challenge, indicating its involvement in the immune response of clam. Yeast two-hybrid assays verified that MpTAF5L can interact with MpMITF (a critical immune-related transcription factor), and our further research clarified this interaction depended upon the N-terminal TAF5-NTD2 domain of MpTAF5L. Moreover, the mRNA expression of MpBcl-2 (a target gene of MITF) was significantly decreased but the mRNA expression of MpMITF was not significantly changed after knockdown of MpTAF5L, which indicated the reduction of MpMITF regulating activity at the same time. These results revealed that MpTAF5L interacted with MpMITF and enhanced the activation of MpMITF, which plays roles in the immune defense against V. parahaemolyticus. |
| 关键词 | TAF5L MITF Innate immunity Meretrix petechialis |
| DOI | 10.1016/j.fsi.2019.11.039 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao); China Agriculture Research System[CARS-49]; National Natural Science Foundation of China[31772845] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000516880900107 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167042 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Baozhong |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Wang, Di,Zhang, Shujing,Liu, Baozhong. TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:1017-1023. |
| APA | Wang, Di,Zhang, Shujing,&Liu, Baozhong.(2020).TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis.FISH & SHELLFISH IMMUNOLOGY,98,1017-1023. |
| MLA | Wang, Di,et al."TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis".FISH & SHELLFISH IMMUNOLOGY 98(2020):1017-1023. |
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