| High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography | |
Li, Kecheng1,2; Liu, Song1; Xing, Ronge1 ; Yu, Huahua1; Qin, Yukun1; Li, Rongfeng1; Li, Pengcheng1; Li, PC
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| 2013-04-01 | |
| 发表期刊 | JOURNAL OF SEPARATION SCIENCE
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| ISSN | 1615-9306 |
| 卷号 | 36期号:7页码:1275-1282 |
| 文章类型 | Article |
| 摘要 | Highly purified chitooligomers with single degree of polymerization are of significance for studying bioactivity of chitooligomers. However, there are few reports on high-resolution preparative separation of chitooligomers, especially for those oligomers with degree of polymerization higher than 4. This study developed a high-resolution chromatography for the preparative separation of a pure fully deacetylated chitooligomer series. A glucosamine oligomer mixture with low degree of polymerization was prepared by acid hydrolysis of a highly deacetylated chitosan. Then, six fractions were separated from the prepared oligomer mixture by ion-exchange chromatography and analyzed by HPLC and ESI/MS, which primarily contained glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively, with chromatographic purities over 98% for dimers to hexamers and a purity of 93% for heptamers. The yields of a single round of separation were 75, 60, 60, 55, 35, and 20 mg for glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively. Furthermore, a chromatographic separation model for GlcN homomers was established. The capacity factor (k) of glucosamine oligomers and their degrees of polymerization (DPs) exhibited a good correlation, lnk = 0.786 + 0.846 lnDP, (R-2 = 0.997). Based on this equation, glucosamine octamers are expected to be separated by this system.; Highly purified chitooligomers with single degree of polymerization are of significance for studying bioactivity of chitooligomers. However, there are few reports on high-resolution preparative separation of chitooligomers, especially for those oligomers with degree of polymerization higher than 4. This study developed a high-resolution chromatography for the preparative separation of a pure fully deacetylated chitooligomer series. A glucosamine oligomer mixture with low degree of polymerization was prepared by acid hydrolysis of a highly deacetylated chitosan. Then, six fractions were separated from the prepared oligomer mixture by ion-exchange chromatography and analyzed by HPLC and ESI/MS, which primarily contained glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively, with chromatographic purities over 98% for dimers to hexamers and a purity of 93% for heptamers. The yields of a single round of separation were 75, 60, 60, 55, 35, and 20 mg for glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively. Furthermore, a chromatographic separation model for GlcN homomers was established. The capacity factor (k) of glucosamine oligomers and their degrees of polymerization (DPs) exhibited a good correlation, lnk = 0.786 + 0.846 lnDP, (R-2 = 0.997). Based on this equation, glucosamine octamers are expected to be separated by this system. |
| 关键词 | Chitooligomers Chitosan Ion-exchange Chromatography |
| 学科领域 | Chemistry |
| DOI | 10.1002/jssc.201200935 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000319865700016 |
| WOS关键词 | MOLECULAR-WEIGHT ; LIQUID-CHROMATOGRAPHY ; BIOLOGICAL-ACTIVITIES ; HYDROGEN-PEROXIDE ; CHITOSAN ; OLIGOSACCHARIDES ; CHITOOLIGOSACCHARIDES ; OLIGOMERS ; DEGRADATION ; MEDICINE |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16703 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 通讯作者 | Li, PC |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Kecheng,Liu, Song,Xing, Ronge,et al. High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(7):1275-1282. |
| APA | Li, Kecheng.,Liu, Song.,Xing, Ronge.,Yu, Huahua.,Qin, Yukun.,...&Li, PC.(2013).High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography.JOURNAL OF SEPARATION SCIENCE,36(7),1275-1282. |
| MLA | Li, Kecheng,et al."High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography".JOURNAL OF SEPARATION SCIENCE 36.7(2013):1275-1282. |
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