Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Transcriptome analysis reveals the regulation of the shrimp STAT on host chitin-binding domain containing proteins and energy metabolism process during WSSV infection | |
Yang, Feifei1,2,3,4; Li, Shihao1,2,3; Xiang, Jianhai1,2 ; Zhao, Xingming5; Li, Fuhua1,2,3
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| 2020-05-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 100页码:345-357 |
| 通讯作者 | Li, Shihao([email protected]) |
| 摘要 | JAK/STAT signaling pathway is suggested to enhance the infection of WSSV in crustaceans. However, the regulation mechanism of this process is not quite clear. Here, comparative transcriptomic analysis was performed among shrimps before and after Litopenaeus vannamei STAT (LvSTAT) was silenced by dsRNA approach during WSSV infection. Differentially expressed genes (DEGs) common in the STAT-interfered groups and control groups at different times after WSSV infection were analyzed to acquire the genes probably regulated by LvSTAT. DEGs annotation and further GO terms enrichment analyses revealed that the identified DEGs mainly contained two categories, chitin-binding domain containing proteins and energy metabolism related genes. The former mainly included cuticle proteins, thrombospondins (TSPs) and peritrophin, while the later mainly included hexose catabolic process and glycolysis related genes. Two cuticle proteins and two TSPs were further studied to learn their expression changes during WSSV infection. They were significantly regulated during WSSV infection, implying the involvement of chitin-binding domain containing protein in the invasion process of WSSV. Systematic analysis on the glycolysis and lipid synthesis pathway demonstrated that silencing of LvSTAT could reduce the glycolysis efficiency and the production of lipids. It could be speculated that a favorable function of LvSTAT for WSSV replication existed by regulating the energy metabolism of the host. Through revealing the main category of genes and biological processes regulated by STAT, our study could shed new light on the roles of JAK/STAT signaling pathway in shrimp during virus infection. |
| 关键词 | STAT WSSV Chitin binding Glycolysis Lipid metabolism Litopenaeus vannamei |
| DOI | 10.1016/j.fsi.2020.03.026 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | China Agriculture Research System-48[CARS-48]; China Postdoctoral Science Foundation[2017M612361]; General Program of National Natural Science Foundation of China[31772880]; General Program of National Natural Science Foundation of China[41776158] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000523603600037 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167027 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, Shihao |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Lianyungang 222005, Peoples R China 5.Fudan Univ, Inst Sci & Technol Brain Inspired Intelligence, Shanghai 200433, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Yang, Feifei,Li, Shihao,Xiang, Jianhai,et al. Transcriptome analysis reveals the regulation of the shrimp STAT on host chitin-binding domain containing proteins and energy metabolism process during WSSV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2020,100:345-357. |
| APA | Yang, Feifei,Li, Shihao,Xiang, Jianhai,Zhao, Xingming,&Li, Fuhua.(2020).Transcriptome analysis reveals the regulation of the shrimp STAT on host chitin-binding domain containing proteins and energy metabolism process during WSSV infection.FISH & SHELLFISH IMMUNOLOGY,100,345-357. |
| MLA | Yang, Feifei,et al."Transcriptome analysis reveals the regulation of the shrimp STAT on host chitin-binding domain containing proteins and energy metabolism process during WSSV infection".FISH & SHELLFISH IMMUNOLOGY 100(2020):345-357. |
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