Assessment of introduced Kappaphycus (Solieriaceae, Rhodophyta) species relationships in China with molecular markers | |
Liu Chenlin1; Huang Xiaohang1; Liu Jianguo2; Liu, CL | |
2013-02-01 | |
发表期刊 | ACTA OCEANOLOGICA SINICA
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ISSN | 0253-505X |
卷号 | 32期号:2页码:59-65 |
文章类型 | Article |
摘要 | Due to morphological plasticity and paucity of diagnostic morphological characters, the taxonomy of Kappaphycus gets more and more confused with the expanding of commercial cultivation. In this study, the phylogenetic relationship of 13 strains of introduced Kappaphycus species in China was defined using DNA molecular markers, such as 18S rDNA, rbcL and cox2-cox3 spacer region. The resolutions obtained by three different molecular markers were compared: both cox2-cox3 spacer region and rbcL sequences are eligible in inter- species identification of Kappaphycus, whereas cox2-cox3 spacer region is more variable than rbcL sequence. There is several basepairs' discrepancy among 18S rDNA sequences, while it is 100% identical among both cox2-cox3 spacer region and rbcL sequences of the ten strains of K. alvarezii. We suppose that 18S rDNA sequence can provide more information in biogeography study of Kappaphycus than other two DNA sequences.; Due to morphological plasticity and paucity of diagnostic morphological characters, the taxonomy of Kappaphycus gets more and more confused with the expanding of commercial cultivation. In this study, the phylogenetic relationship of 13 strains of introduced Kappaphycus species in China was defined using DNA molecular markers, such as 18S rDNA, rbcL and cox2-cox3 spacer region. The resolutions obtained by three different molecular markers were compared: both cox2-cox3 spacer region and rbcL sequences are eligible in inter- species identification of Kappaphycus, whereas cox2-cox3 spacer region is more variable than rbcL sequence. There is several basepairs' discrepancy among 18S rDNA sequences, while it is 100% identical among both cox2-cox3 spacer region and rbcL sequences of the ten strains of K. alvarezii. We suppose that 18S rDNA sequence can provide more information in biogeography study of Kappaphycus than other two DNA sequences. |
关键词 | 18s Rdna Cox2-cox3 Spacer Region Molecular Marker Kappaphycus Rbcl |
学科领域 | Oceanography |
DOI | 10.1007/s13131-013-0277-5 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Oceanography |
WOS类目 | Oceanography |
WOS记录号 | WOS:000314699900008 |
WOS关键词 | RBCL ; SEQUENCES ; GIGARTINALES ; SYSTEMATICS ; RDNA |
WOS标题词 | Science & Technology ; Physical Sciences |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/16684 |
专题 | 海洋生物技术研发中心 |
通讯作者 | Liu, CL |
作者单位 | 1.State Ocean Adm, Inst Oceanog 1, Marine Ecol Ctr, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Liu Chenlin,Huang Xiaohang,Liu Jianguo,et al. Assessment of introduced Kappaphycus (Solieriaceae, Rhodophyta) species relationships in China with molecular markers[J]. ACTA OCEANOLOGICA SINICA,2013,32(2):59-65. |
APA | Liu Chenlin,Huang Xiaohang,Liu Jianguo,&Liu, CL.(2013).Assessment of introduced Kappaphycus (Solieriaceae, Rhodophyta) species relationships in China with molecular markers.ACTA OCEANOLOGICA SINICA,32(2),59-65. |
MLA | Liu Chenlin,et al."Assessment of introduced Kappaphycus (Solieriaceae, Rhodophyta) species relationships in China with molecular markers".ACTA OCEANOLOGICA SINICA 32.2(2013):59-65. |
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