Development of SSR primers from EST sequences and their application in germplasm identification of Porphyra lines (Rhodophyta)

doi:10.1080/09670260600740906

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	Development of SSR primers from EST sequences and their application in germplasm identification of Porphyra lines (Rhodophyta)
	Sun, Jianwei ; Liu, Tao ; Gu, Baotai ; Jin, Demin ; Weng, Manli ; Feng, Yanbin ; Xu, Pu ; Duan, Delin ; Wang, Bin; Wang, B, Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
	2006-08-01
发表期刊	EUROPEAN JOURNAL OF PHYCOLOGY
ISSN	0967-0262
卷号	41 期号:3 页码:329-336
文章类型	Article
摘要	This paper reports the development of SSR markers from EST data and their utilization in germplasm identification of Porphyra. The publicly available EST (expressed sequence tag) sequences of Porphyra were searched from the Internet (www.kazura.or.jp/en/plant/porphyra/EST/). From a total of 20,779 obtained EST sequences, 391 SSRs (simple sequence repeats) were analysed with SSRIT software (www.gramene.org/db/searches/ssrtool). From those, 48 SSR primer-pairs were designed and tested by commonly used SSR reaction conditions using 22 Porphyra DNA samples as templates. Results showed that 41 SSR primer-pairs gave good amplification patterns. These were used to conduct SSR analyses of genetic diversity and variety identification of the 22 Porphyra lines. A dendrogram and the DNA fingerprints of the Porphyra lines were developed based on the obtained SSR data.; This paper reports the development of SSR markers from EST data and their utilization in germplasm identification of Porphyra. The publicly available EST (expressed sequence tag) sequences of Porphyra were searched from the Internet (www.kazura.or.jp/en/plant/porphyra/EST/). From a total of 20,779 obtained EST sequences, 391 SSRs (simple sequence repeats) were analysed with SSRIT software (www.gramene.org/db/searches/ssrtool). From those, 48 SSR primer-pairs were designed and tested by commonly used SSR reaction conditions using 22 Porphyra DNA samples as templates. Results showed that 41 SSR primer-pairs gave good amplification patterns. These were used to conduct SSR analyses of genetic diversity and variety identification of the 22 Porphyra lines. A dendrogram and the DNA fingerprints of the Porphyra lines were developed based on the obtained SSR data.
关键词	Cssr Dna Fingerprint Microsatellite Porphyra Seaweeds Simple Sequence Repeats (Ssrs)
学科领域	Plant Sciences ; Marine & Freshwater Biology
DOI	10.1080/09670260600740906
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000240755200007
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1667
专题	实验海洋生物学重点实验室
通讯作者	Wang, B, Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Laiyang Agr Coll, Fac Life Sci, Laiyang 265200, Peoples R China 3.Ocean Univ China, Minist Educ, Key Lab Mariculture, Qingdao 266003, Peoples R China 4.Jiangsu Inst Oceanol & Hydrol, Lucknow 226007, Uttar Pradesh, India 5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jianwei,Liu, Tao,Gu, Baotai,et al. Development of SSR primers from EST sequences and their application in germplasm identification of Porphyra lines (Rhodophyta)[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2006,41(3):329-336.
APA	Sun, Jianwei.,Liu, Tao.,Gu, Baotai.,Jin, Demin.,Weng, Manli.,...&Wang, B, Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China.(2006).Development of SSR primers from EST sequences and their application in germplasm identification of Porphyra lines (Rhodophyta).EUROPEAN JOURNAL OF PHYCOLOGY,41(3),329-336.
MLA	Sun, Jianwei,et al."Development of SSR primers from EST sequences and their application in germplasm identification of Porphyra lines (Rhodophyta)".EUROPEAN JOURNAL OF PHYCOLOGY 41.3(2006):329-336.

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