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| MiR-125b acts as an oncogene in glioblastoma cells and inhibits cell apoptosis through p53 and p38MAPK-independent pathways | |
Wu, N.1; Lin, X.2; Zhao, X.3 ; Zheng, L.4; Xiao, L.1,5; Liu, J.1; Ge, L.1; Cao, S.6; Lin, X
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| 2013-11-26 | |
| 发表期刊 | BRITISH JOURNAL OF CANCER
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| ISSN | 0007-0920 |
| 卷号 | 109期号:11页码:2853-2863 |
| 文章类型 | Article |
| 摘要 | Background: We have recently identified miR-125b upregulation in glioblastoma (GMB). The aim of this study is to determine the correlation between miR-125b expression and malignant grades of glioma and the genes targeted by miR-125b.; Background: We have recently identified miR-125b upregulation in glioblastoma (GMB). The aim of this study is to determine the correlation between miR-125b expression and malignant grades of glioma and the genes targeted by miR-125b. Methods: Real-time PCR was employed to measure the expression level of miR-125b. Cell viability was evaluated by cell growth and colony formation in soft-agar assays. Cell apoptosis was determined by Hoechst 33342 staining and AnnexinV-FITC assay. The Luciferase assay was used to confirm the actual binding sites of p38MAPK mRNA. Western blot was used to detect the gene expression level. Results: The expression level of miR-125b is positively correlated with the malignant grade of glioma. Ectopic expression of miR-125b promotes the proliferation of GMB cells. Knockdown of endogenous miR-125b inhibits cell proliferation and promotes cell apoptosis. Further studies reveal that p53 is regulated by miR-125b. However, downregulation of the endogenous miR-125b also results in p53-independent apoptotic pathway leading to apoptosis in p53 mutated U251 cells and p53 knockdown U87 cells. Moreover, p38MAPK is also regulated by miR-125b and downregulation of miR-125b activates the p38MAPK-induced mitochondria apoptotic pathway. Conclusion: High-level expression of miR-125b is associated with poor outcomes of GMB. MiR-125b may have an oncogenic role in GMB cells by promoting cell proliferation and inhibiting apoptosis. |
| 关键词 | Microrna Mir-125b Glioblastoma Cell Apoptosis P53 P38mapk |
| 学科领域 | Oncology |
| DOI | 10.1038/bjc.2013.672 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Oncology |
| WOS类目 | Oncology |
| WOS记录号 | WOS:000327762700014 |
| WOS关键词 | ACTIVATED PROTEIN-KINASES ; ANTAGONIST KILLER 1 ; NEURONAL DIFFERENTIATION ; MICRORNA SIGNATURES ; OVARIAN-CANCER ; STEM-CELLS ; EXPRESSION ; CONFERS ; PROLIFERATION ; TEMOZOLOMIDE |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16678 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Lin, X |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China 3.Qingdao Univ, Cent Lab, Affiliated Hosp, Coll Med, Qingdao 266003, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 5.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Peoples R China 6.Roswell Pk Canc Inst, Dept Med, Buffalo, NY 14263 USA |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wu, N.,Lin, X.,Zhao, X.,et al. MiR-125b acts as an oncogene in glioblastoma cells and inhibits cell apoptosis through p53 and p38MAPK-independent pathways[J]. BRITISH JOURNAL OF CANCER,2013,109(11):2853-2863. |
| APA | Wu, N..,Lin, X..,Zhao, X..,Zheng, L..,Xiao, L..,...&Lin, X.(2013).MiR-125b acts as an oncogene in glioblastoma cells and inhibits cell apoptosis through p53 and p38MAPK-independent pathways.BRITISH JOURNAL OF CANCER,109(11),2853-2863. |
| MLA | Wu, N.,et al."MiR-125b acts as an oncogene in glioblastoma cells and inhibits cell apoptosis through p53 and p38MAPK-independent pathways".BRITISH JOURNAL OF CANCER 109.11(2013):2853-2863. |
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