Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis

doi:10.1371/journal.pone.0060740

IOCAS-IR > 实验海洋生物学重点实验室

	Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis
	Wu, Xiaojie 1; Huang, Aiyou 2,3; Xu, Meiling 1; Wang, Chao 2,3; Jia, Zhaojun 1; Wang, Guangce 1,2; Niu, Jianfeng 2; Wang, GC
	2013-04-18
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:4 页码:e60740
文章类型	Article
摘要	In order to identify the optimal internal control for relative real-time PCR when studying target gene expression in the red alga Porphyra yezoensis, we quantified the expression of seven housekeeping genes (18S ribosomal RNA, 30S ribosomal protein S8, Polyubiquitin-2, Glyceraldehyde-3-phosphate dehydrogenase, Elongation factor 1-alpha, Beta-tubulin and Actin 3) at different life-history stages. Absolute quantification was done by normalization to total RNA quantity and by normalization to genomic DNA quantity. We used these two normalization approaches, comparing the differences of expression levels of all candidate housekeeping genes between any two generations and across three life-history stages (filamentous sporophytes, leafy gametophytes and conchospores). We found GAPDH had the best stability in all cases and we recommend that GAPDH be considered as a potential internal control for gene expression studies at different life-history stages in P. yezoensis.; In order to identify the optimal internal control for relative real-time PCR when studying target gene expression in the red alga Porphyra yezoensis, we quantified the expression of seven housekeeping genes (18S ribosomal RNA, 30S ribosomal protein S8, Polyubiquitin-2, Glyceraldehyde-3-phosphate dehydrogenase, Elongation factor 1-alpha, Beta-tubulin and Actin 3) at different life-history stages. Absolute quantification was done by normalization to total RNA quantity and by normalization to genomic DNA quantity. We used these two normalization approaches, comparing the differences of expression levels of all candidate housekeeping genes between any two generations and across three life-history stages (filamentous sporophytes, leafy gametophytes and conchospores). We found GAPDH had the best stability in all cases and we recommend that GAPDH be considered as a potential internal control for gene expression studies at different life-history stages in P. yezoensis.
学科领域	Science & Technology - Other Topics
DOI	10.1371/journal.pone.0060740
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000317908700006
WOS关键词	REAL-TIME PCR ; POLYMERASE-CHAIN-REACTION ; RT-PCR ; MESSENGER-RNA ; RIBOSOMAL-RNA ; NORMALIZATION ; RHODOPHYTA ; QUANTIFICATION ; VALIDATION ; BANGIALES
WOS标题词	Science & Technology
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16675
专题	实验海洋生物学重点实验室
通讯作者	Wang, GC
作者单位	1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wu, Xiaojie,Huang, Aiyou,Xu, Meiling,et al. Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis[J]. PLOS ONE,2013,8(4):e60740.
APA	Wu, Xiaojie.,Huang, Aiyou.,Xu, Meiling.,Wang, Chao.,Jia, Zhaojun.,...&Wang, GC.(2013).Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis.PLOS ONE,8(4),e60740.
MLA	Wu, Xiaojie,et al."Variation of Expression Levels of Seven Housekeeping Genes at Different Life-History Stages in Porphyra yezoensis".PLOS ONE 8.4(2013):e60740.

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