Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The response of mRNA expression upon secondary challenge with Vibrio anguillarum suggests the involvement of C-lectins in the immune priming of scallop Chlamys farreri | |
Wang, Jingjing1,2 ; Wang, Lingling1; Yang, Chuanyan1,2; Jiang, Qiufen1,2; Zhang, Huan1; Yue, Feng1,2; Huang, Mengmeng1,2; Sun, Zhibin1; Song, Linsheng1; Wang, LL
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| 2013-06-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| 卷号 | 40期号:2页码:142-147 |
| 文章类型 | Article |
| 摘要 | The enhanced immunity against a second encounter with the particular pathogen has suggested the presence of "immune priming" in scallop. In the present study, the survival rate and expression patterns of five C-lectin isoforms from scallop Chlamys farreri were explored after "vaccination" of heat-killed Vibrio anguillarum or successively challenge with V. anguillarum and Micrococcus luteus. When scallops were challenged with live bacteria, the survival rate increased significantly only in the group firstly "vaccinated" with inactivated V. anguillarum and then challenged with live V. anguillarum compared with naive scallops (from 41% to 63.6%, P < 0.05), showing enhanced protective effects of inactivated bacteria with "specificity". When scallops received the challenge with V. anguillarum, the mRNA expression level of five C-lectins in scallops which were immuned previously with heat-killed V. anguillarum peaked significantly higher (26.7-, 121.7-, 60.1-, 27.4-, 16.3-fold to Oh, respectively, P < 0.01) than that in non-immuned scallops (7.6-fold, P < 0.05; 6.4-, 3.9-fold, P > 0.05; 5.7-fold, P < 0.05; 11.7-fold, P < 0.01, to Oh, respectively). A significantly higher peak and 3-9 h earlier response of all C-lectins mRNA expression were observed after challenge with live V. anguillarum (26.7-, 121.7-, 60.1-, 26.4- and 16.3-fold to Oh, respectively, P < 0.01), compared with those only received first injection with heat-killed V. anguillarum (1.6-fold, P > 0.05; 8.3-fold, P < 0.05; 5.2-fold, P > 0.05; 14.5-fold, P < 0.01; 4.3-fold, P > 0.05, to 0 h, respectively). The response of mRNA expression to the secondary encounter with the same bacteria was stronger than that of successively encounter with the different class of bacteria. It was obvious that the mRNA expression of C-lectins in scallops was significantly enhanced by the successive challenge of same species of bacteria with a certain degree of specificity. All the results suggested that C-lectins might be involved in some form of immune priming, and it might provide new insights into mechanism of invertebrate immune priming. (C) 2013 Elsevier Ltd. All rights reserved.; The enhanced immunity against a second encounter with the particular pathogen has suggested the presence of "immune priming" in scallop. In the present study, the survival rate and expression patterns of five C-lectin isoforms from scallop Chlamys farreri were explored after "vaccination" of heat-killed Vibrio anguillarum or successively challenge with V. anguillarum and Micrococcus luteus. When scallops were challenged with live bacteria, the survival rate increased significantly only in the group firstly "vaccinated" with inactivated V. anguillarum and then challenged with live V. anguillarum compared with naive scallops (from 41% to 63.6%, P < 0.05), showing enhanced protective effects of inactivated bacteria with "specificity". When scallops received the challenge with V. anguillarum, the mRNA expression level of five C-lectins in scallops which were immuned previously with heat-killed V. anguillarum peaked significantly higher (26.7-, 121.7-, 60.1-, 27.4-, 16.3-fold to Oh, respectively, P < 0.01) than that in non-immuned scallops (7.6-fold, P < 0.05; 6.4-, 3.9-fold, P > 0.05; 5.7-fold, P < 0.05; 11.7-fold, P < 0.01, to Oh, respectively). A significantly higher peak and 3-9 h earlier response of all C-lectins mRNA expression were observed after challenge with live V. anguillarum (26.7-, 121.7-, 60.1-, 26.4- and 16.3-fold to Oh, respectively, P < 0.01), compared with those only received first injection with heat-killed V. anguillarum (1.6-fold, P > 0.05; 8.3-fold, P < 0.05; 5.2-fold, P > 0.05; 14.5-fold, P < 0.01; 4.3-fold, P > 0.05, to 0 h, respectively). The response of mRNA expression to the secondary encounter with the same bacteria was stronger than that of successively encounter with the different class of bacteria. It was obvious that the mRNA expression of C-lectins in scallops was significantly enhanced by the successive challenge of same species of bacteria with a certain degree of specificity. All the results suggested that C-lectins might be involved in some form of immune priming, and it might provide new insights into mechanism of invertebrate immune priming. (C) 2013 Elsevier Ltd. All rights reserved. |
| 关键词 | C-lectin Immune Priming Chlamys Farreri Vibrio Anguillarum |
| 学科领域 | Immunology ; Zoology |
| DOI | 10.1016/j.dci.2013.02.003 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Immunology ; Zoology |
| WOS类目 | Immunology ; Zoology |
| WOS记录号 | WOS:000320491100007 |
| WOS关键词 | INNATE IMMUNITY ; INVERTEBRATE IMMUNITY ; PATHOGEN RECOGNITION ; PROTECTION ; MEMORY ; SPECIFICITY ; DROSOPHILA ; DEFENSE ; CFLEC-2 ; SYSTEMS |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16658 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, LL |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Jingjing,Wang, Lingling,Yang, Chuanyan,et al. The response of mRNA expression upon secondary challenge with Vibrio anguillarum suggests the involvement of C-lectins in the immune priming of scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,40(2):142-147. |
| APA | Wang, Jingjing.,Wang, Lingling.,Yang, Chuanyan.,Jiang, Qiufen.,Zhang, Huan.,...&Wang, LL.(2013).The response of mRNA expression upon secondary challenge with Vibrio anguillarum suggests the involvement of C-lectins in the immune priming of scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,40(2),142-147. |
| MLA | Wang, Jingjing,et al."The response of mRNA expression upon secondary challenge with Vibrio anguillarum suggests the involvement of C-lectins in the immune priming of scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 40.2(2013):142-147. |
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