Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein | |
Wang, Mengqiang1 ; Zhang, Daoxiang1,2; Wang, Lingling1; Gai, Yunchao1; Zhou, Zhi1; Zhang, Huan1; Song, Linsheng1; Song, LS
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| 2013-03-15 | |
| 发表期刊 | ELECTRONIC JOURNAL OF BIOTECHNOLOGY
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| ISSN | 0717-3458 |
| 卷号 | 16期号:2页码:5 |
| 文章类型 | Article |
| 摘要 | Background: Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds and interacts with a variety of proteins to regulate their activities.; Background: Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds and interacts with a variety of proteins to regulate their activities. Results: The full-length cDNA of crab Eriocheir sinensis CypA (EsCypA) was cloned by EST and RACE technique. The complete sequence of EsCypA cDNA contained a 5' untranslated region (UTR) of 50 bp, a 3' UTR of 233 bp with a polyA tail, and an open reading frame (ORF) of 495 bp encoding a polypeptide of 164 amino acids with the predicted molecular weight of 17.36 kDa. The deduced amino acid sequence of EsCypA contained two highly conserved signature sequences of peptidyl-prolyl cis-trans isomerase and a pro-isomerase domain. The mRNA transcripts of EsCypA were detectable in all the examined tissues, including haemocytes, gill, hepatopancreas, gonad, muscle and heart, with higher expression level in hepatopancreas and gonad. No significant difference in the relative mRNA expression level of EsCypA was observed during the whole course of bacteria challenge, whereas it was up-regulated during fungi challenge. The purified recombinant protein rEsCypA exhibited a significant PPIase activity and an antifungal activity. Conclusions: All these results indicated that it was a typical CypA member and potentially involved in the innate immune responses of crab. |
| 关键词 | Antifungal Activity Cyclophilin a Eriocheir Sinensis Immune Response Ppiase Activity |
| 学科领域 | Biotechnology & Applied Microbiology |
| DOI | 10.2225/vol16-issue2-fulltext-5 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000318349300006 |
| WOS关键词 | CIS-TRANS ISOMERASE ; ACTIVATED T-CELLS ; SIGNALING PATHWAYS ; CHLAMYS-FARRERI ; BINDING-PROTEIN ; NUCLEAR FACTOR ; CLONING ; IMMUNOPHILINS ; HIV-1 ; GENE |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16654 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Song, LS |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Mengqiang,Zhang, Daoxiang,Wang, Lingling,et al. The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2013,16(2):5. |
| APA | Wang, Mengqiang.,Zhang, Daoxiang.,Wang, Lingling.,Gai, Yunchao.,Zhou, Zhi.,...&Song, LS.(2013).The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,16(2),5. |
| MLA | Wang, Mengqiang,et al."The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 16.2(2013):5. |
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