The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis

doi:10.1111/iji.12019

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	The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis
	Wang, M.1; Wang, L.1 ; Zhou, Z.1,2; Gao, Y.1,2 ; Wang, L.1,2 ; Shi, X.1,2; Gai, Y.1; Mu, C.1; Song, L.1 ; Wang, L
	2013-06-01
发表期刊	INTERNATIONAL JOURNAL OF IMMUNOGENETICS
ISSN	1744-3121
卷号	40 期号:3 页码:230-240
文章类型	Article
摘要	Catalase (CAT) is an antioxidant enzyme and plays a significant role in the protection against oxidative stress by reducing hydrogen peroxide. The CAT cDNA of Eriocheir sinensis (EsCAT) was cloned via RACE technique. The complete sequence of EsCAT cDNA consisted of a 5 untranslated regions (UTR) of 224bp, a 3 UTR of 1287bp with a poly (A) tail and an open reading frame (ORF) of 1542bp, which encoded a polypeptide of 513 amino acid residues with a calculated molecular mass of approximately 58.86kDa and a theoretical isoelectric point of 6.880. The deduced amino acid sequence of EsCAT contained a highly conserved proximal active-site signature motif (60FDRERIPERVVHAKGAL76) and a proximal hemeligand signature motif (350RLFSYNDTH358) and exhibited high similarity with other reported CATs. In the phylogenetic tree, EsCAT was clustered with the CATs from Scylla serrata and Portunus trituberculatus. The EsCAT transcripts were constitutively expressed in haepatopancreas, haemocytes, gill, gonad, muscle and heart, with highest expression level in haepatopancreas. The relative expression level of EsCAT mRNA in haemocytes was continuously up-regulated and reached the peak level at 48h post-Vibrio anguillarum challenge. The purified recombinant EsCAT protein displayed antioxidant activity against hydrogen peroxide with high thermal stability and broad spectrum of pH values. All these results demonstrated that EsCAT was an efficient antioxidant enzyme and potentially involved in the regulation of redox and innate immune response of crabs.; Catalase (CAT) is an antioxidant enzyme and plays a significant role in the protection against oxidative stress by reducing hydrogen peroxide. The CAT cDNA of Eriocheir sinensis (EsCAT) was cloned via RACE technique. The complete sequence of EsCAT cDNA consisted of a 5 untranslated regions (UTR) of 224bp, a 3 UTR of 1287bp with a poly (A) tail and an open reading frame (ORF) of 1542bp, which encoded a polypeptide of 513 amino acid residues with a calculated molecular mass of approximately 58.86kDa and a theoretical isoelectric point of 6.880. The deduced amino acid sequence of EsCAT contained a highly conserved proximal active-site signature motif (60FDRERIPERVVHAKGAL76) and a proximal hemeligand signature motif (350RLFSYNDTH358) and exhibited high similarity with other reported CATs. In the phylogenetic tree, EsCAT was clustered with the CATs from Scylla serrata and Portunus trituberculatus. The EsCAT transcripts were constitutively expressed in haepatopancreas, haemocytes, gill, gonad, muscle and heart, with highest expression level in haepatopancreas. The relative expression level of EsCAT mRNA in haemocytes was continuously up-regulated and reached the peak level at 48h post-Vibrio anguillarum challenge. The purified recombinant EsCAT protein displayed antioxidant activity against hydrogen peroxide with high thermal stability and broad spectrum of pH values. All these results demonstrated that EsCAT was an efficient antioxidant enzyme and potentially involved in the regulation of redox and innate immune response of crabs.
学科领域	Genetics & Heredity ; Immunology
DOI	10.1111/iji.12019
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Genetics & Heredity ; Immunology
WOS类目	Genetics & Heredity ; Immunology
WOS记录号	WOS:000318810200010
WOS关键词	BEEF-LIVER CATALASE ; REACTIVE OXYGEN ; INNATE IMMUNITY ; SACCHAROMYCES-CEREVISIAE ; ANTIOXIDANT ENZYMES ; OLD ENZYME ; GENE ; CLONING ; EXPRESSION ; DAMAGE
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16653
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
通讯作者	Wang, L
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, M.,Wang, L.,Zhou, Z.,et al. The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis[J]. INTERNATIONAL JOURNAL OF IMMUNOGENETICS,2013,40(3):230-240.
APA	Wang, M..,Wang, L..,Zhou, Z..,Gao, Y..,Wang, L..,...&Wang, L.(2013).The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis.INTERNATIONAL JOURNAL OF IMMUNOGENETICS,40(3),230-240.
MLA	Wang, M.,et al."The molecular characterization of a catalase from Chinese mitten crab Eriocheir sinensis".INTERNATIONAL JOURNAL OF IMMUNOGENETICS 40.3(2013):230-240.

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