The megalocytivirus-induced protein CsMig1 enhances Cynoglossus semilaevis resistance against viral infection

doi:10.1016/j.vetimm.2012.11.005

IOCAS-IR > 实验海洋生物学重点实验室

	The megalocytivirus-induced protein CsMig1 enhances Cynoglossus semilaevis resistance against viral infection
	Wang, Wei 1,2; Xiao, Zhi-zhong 1; Sun, Li 1; Sun, L
	2013-01-15
发表期刊	VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
ISSN	0165-2427
卷号	151 期号:1-2 页码:173-179
文章类型	Article
摘要	Half-smooth tongue sole (Cynoglossus semilaevis) is an important economic fish species cultured in northern China. In this study, we identified and analyzed the expression and function of a megalocytivirus-induced gene, CsMig1, from tongue sole. The deduced amino acid sequence of CsMig1 is composed of 507 residues and contains no conserved domains. Blast analysis identified no close homologues of CsMig1. CsMig1 shares moderate sequence similarities in the N-terminal region with the Gig1 (i.e., grass carp hemorrhagic virus-induced gene) homologues of several teleost species. Quantitative real time RTPCR analysis showed that constitutive CsMig1 expression occurred, in increasing order, in heart, spleen, muscle, kidney, liver, gill, and gut. Experimental infection with the viral pathogen megalocytivirus upregulated CsMig1 expression in kidney, spleen, and liver in time-dependent manners. Treatment of head kidney lymphocytes with the culture supernatant of megalocytivirus-stimulated cells significantly enhanced CsMig1 expression. When head kidney lymphocytes were transfected with the Plasmid that constitutively expresses CsMig1, the cells exhibited significantly increased ability to resist megalocytivirus infection. Taken together, these results indicate that CsMig1 is a virus- and, possibly, interferon-induced novel immune factor that functions in the antiviral immunity of tongue sole. (C) 2012 Elsevier B.V. All rights reserved.; Half-smooth tongue sole (Cynoglossus semilaevis) is an important economic fish species cultured in northern China. In this study, we identified and analyzed the expression and function of a megalocytivirus-induced gene, CsMig1, from tongue sole. The deduced amino acid sequence of CsMig1 is composed of 507 residues and contains no conserved domains. Blast analysis identified no close homologues of CsMig1. CsMig1 shares moderate sequence similarities in the N-terminal region with the Gig1 (i.e., grass carp hemorrhagic virus-induced gene) homologues of several teleost species. Quantitative real time RTPCR analysis showed that constitutive CsMig1 expression occurred, in increasing order, in heart, spleen, muscle, kidney, liver, gill, and gut. Experimental infection with the viral pathogen megalocytivirus upregulated CsMig1 expression in kidney, spleen, and liver in time-dependent manners. Treatment of head kidney lymphocytes with the culture supernatant of megalocytivirus-stimulated cells significantly enhanced CsMig1 expression. When head kidney lymphocytes were transfected with the Plasmid that constitutively expresses CsMig1, the cells exhibited significantly increased ability to resist megalocytivirus infection. Taken together, these results indicate that CsMig1 is a virus- and, possibly, interferon-induced novel immune factor that functions in the antiviral immunity of tongue sole. (C) 2012 Elsevier B.V. All rights reserved.
关键词	Cynoglossus Semilaevis Megalocytivirus Interferon Antiviral
学科领域	Immunology ; Veterinary Sciences
DOI	10.1016/j.vetimm.2012.11.005
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Immunology ; Veterinary Sciences
WOS类目	Immunology ; Veterinary Sciences
WOS记录号	WOS:000314012700019
WOS关键词	PANCREATIC NECROSIS VIRUS ; DOUBLE-STRANDED-RNA ; PARALICHTHYS-OLIVACEUS ; TELEOST FISH ; ANTIVIRAL RESPONSES ; BIOLOGICAL-ACTIVITY ; JAPANESE FLOUNDER ; I INTERFERON ; EXPRESSION ; GENES
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16652
专题	实验海洋生物学重点实验室
通讯作者	Sun, L
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Wei,Xiao, Zhi-zhong,Sun, Li,et al. The megalocytivirus-induced protein CsMig1 enhances Cynoglossus semilaevis resistance against viral infection[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2013,151(1-2):173-179.
APA	Wang, Wei,Xiao, Zhi-zhong,Sun, Li,&Sun, L.(2013).The megalocytivirus-induced protein CsMig1 enhances Cynoglossus semilaevis resistance against viral infection.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,151(1-2),173-179.
MLA	Wang, Wei,et al."The megalocytivirus-induced protein CsMig1 enhances Cynoglossus semilaevis resistance against viral infection".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 151.1-2(2013):173-179.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
The megalocytivirus-（1037KB）			限制开放	CC BY-NC-SA	浏览