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| The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection | |
Wang, Chong1,2 ; Hu, Yong-hua1; Chi, Heng1; Sun, Li1; Sun, L
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| 2013-09-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 35期号:3页码:858-865 |
| 文章类型 | Article |
| 摘要 | Edwardsiella tarda is a Gram-negative bacterium that is reckoned one of the most severe fish pathogens. In this study, we analyzed the biological properties of the E. tarda major fimbrial subunit protein, FimA. We found that mutation of fimA resulted in defective biofilm growth, attenuated infectivity against host cells, and impaired ability to disseminate into and colonize host tissues following experimental infection. When used as a subunit vaccine, recombinant FimA (rFimA) elicited a high level of protection in turbot (Scophthalmus maximus) against lethal E. tarda challenge. Immunological analysis showed that rFimA vaccination induced production of specific serum antibodies that bound to live E. tarda via interaction with the FimA on bacterial cells, and that antibody-E. tarda interaction blocked bacterial infection. Furthermore, passive immunization of turbot with anti-rFimA serum before E. tarda infection reduced bacterial loads in fish tissues to significant extents. To examine the adjuvant potential of FimA, turbot were vaccinated with rVhhP2, a protective Vibrio harveyi antigen, in the presence or absence of rFimA. Subsequent analysis showed that the presence of rFimA significantly augmented the protectivity of rVhhP2. ELISA and quantitative real time RT-PCR showed that rFimA significantly increased rVhhP2-specific serum antibody production and enhanced the expression of immune relevant genes. Taken together, these results indicate that FimA is a virulence-associated protein that possesses vaccine as well as adjuvant potentials, and that the immunoprotectivity of FimA is most likely due to its ability to induce specific immune response that inhibits E. tarda infection. (C) 2013 Elsevier Ltd. All rights reserved.; Edwardsiella tarda is a Gram-negative bacterium that is reckoned one of the most severe fish pathogens. In this study, we analyzed the biological properties of the E. tarda major fimbrial subunit protein, FimA. We found that mutation of fimA resulted in defective biofilm growth, attenuated infectivity against host cells, and impaired ability to disseminate into and colonize host tissues following experimental infection. When used as a subunit vaccine, recombinant FimA (rFimA) elicited a high level of protection in turbot (Scophthalmus maximus) against lethal E. tarda challenge. Immunological analysis showed that rFimA vaccination induced production of specific serum antibodies that bound to live E. tarda via interaction with the FimA on bacterial cells, and that antibody-E. tarda interaction blocked bacterial infection. Furthermore, passive immunization of turbot with anti-rFimA serum before E. tarda infection reduced bacterial loads in fish tissues to significant extents. To examine the adjuvant potential of FimA, turbot were vaccinated with rVhhP2, a protective Vibrio harveyi antigen, in the presence or absence of rFimA. Subsequent analysis showed that the presence of rFimA significantly augmented the protectivity of rVhhP2. ELISA and quantitative real time RT-PCR showed that rFimA significantly increased rVhhP2-specific serum antibody production and enhanced the expression of immune relevant genes. Taken together, these results indicate that FimA is a virulence-associated protein that possesses vaccine as well as adjuvant potentials, and that the immunoprotectivity of FimA is most likely due to its ability to induce specific immune response that inhibits E. tarda infection. (C) 2013 Elsevier Ltd. All rights reserved. |
| 关键词 | Edwardsiella Tarda Fimbrial Virulence Vaccine Adjuvant |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2013.06.021 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000324511700028 |
| WOS关键词 | OUTER-MEMBRANE PROTEIN ; PROTECTIVE IMMUNOGEN ; BIOFILM FORMATION ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; SCOPHTHALMUS-MAXIMUS ; VIBRIO-ANGUILLARUM ; TYPE-1 FIMBRIAE ; URINARY-TRACT ; IDENTIFICATION |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16651 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Chong,Hu, Yong-hua,Chi, Heng,et al. The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):858-865. |
| APA | Wang, Chong,Hu, Yong-hua,Chi, Heng,Sun, Li,&Sun, L.(2013).The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection.FISH & SHELLFISH IMMUNOLOGY,35(3),858-865. |
| MLA | Wang, Chong,et al."The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):858-865. |
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