Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications

doi:10.1111/ppl.12008

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	Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications
	Lin, A-Peng 1; Wang, Guang-Ce 1; Zhou, Wen-Qin 2; Wang, GC
	2013-08-01
发表期刊	PHYSIOLOGIA PLANTARUM
ISSN	0031-9317
卷号	148 期号:4 页码:582-589
文章类型	Article
摘要	Zostera marina (eelgrass) is an important ecological component of many shallow, temperate lagoons. Evidence suggests that Z. marina has a high bicarbonate utilization capability, which could be promoted by possible proton extrusion and the consequent formation of an acid zone' in the apoplastic space (unstirred layer) of its leaves. It has been found that 50mM of the buffer Tris significantly inhibited the photosynthetic O-2 evolution of Z. marina and it was proposed that this was because of Tris's ability to bond with protons outside the cell wall. To investigate if H+ played an important role in the photosynthetic carbon utilization of Z. marina, it is very important to simultaneously monitor the photosynthesis status and possible H+ fluxes. However, probably because of the lack of suitable techniques, this has never been attempted. In this study, experiments were undertaken on Z. marina by monitoring H+ and O-2 fluxes and the relative electron transport rates during light-dark transition. During stable photosynthesis, in addition to an obvious O-2 outflow, there was a significant net H+ influx connected to Z. marina photosynthesis. The inhibitory effects of both Tris and respiration inhibitors on apparent O-2 evolution of Z. marina were confirmed. However, evidence did not support the proposed Tris inhibition mechanism.; Zostera marina (eelgrass) is an important ecological component of many shallow, temperate lagoons. Evidence suggests that Z. marina has a high bicarbonate utilization capability, which could be promoted by possible proton extrusion and the consequent formation of an acid zone' in the apoplastic space (unstirred layer) of its leaves. It has been found that 50mM of the buffer Tris significantly inhibited the photosynthetic O-2 evolution of Z. marina and it was proposed that this was because of Tris's ability to bond with protons outside the cell wall. To investigate if H+ played an important role in the photosynthetic carbon utilization of Z. marina, it is very important to simultaneously monitor the photosynthesis status and possible H+ fluxes. However, probably because of the lack of suitable techniques, this has never been attempted. In this study, experiments were undertaken on Z. marina by monitoring H+ and O-2 fluxes and the relative electron transport rates during light-dark transition. During stable photosynthesis, in addition to an obvious O-2 outflow, there was a significant net H+ influx connected to Z. marina photosynthesis. The inhibitory effects of both Tris and respiration inhibitors on apparent O-2 evolution of Z. marina were confirmed. However, evidence did not support the proposed Tris inhibition mechanism.
学科领域	Plant Sciences
DOI	10.1111/ppl.12008
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000321697300012
WOS关键词	ION-SELECTIVE MICROELECTRODES ; INORGANIC CARBON UTILIZATION ; CHARA-CORALLINA ; PHOTOSYNTHETIC UTILIZATION ; PLASMA-MEMBRANE ; PROTON FLUXES ; SINGLE-CELL ; BICARBONATE ; SEAGRASSES ; TRANSPORT
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16633
专题	实验海洋生物学重点实验室
通讯作者	Wang, GC
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Lin, A-Peng,Wang, Guang-Ce,Zhou, Wen-Qin,et al. Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications[J]. PHYSIOLOGIA PLANTARUM,2013,148(4):582-589.
APA	Lin, A-Peng,Wang, Guang-Ce,Zhou, Wen-Qin,&Wang, GC.(2013).Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications.PHYSIOLOGIA PLANTARUM,148(4),582-589.
MLA	Lin, A-Peng,et al."Simultaneous measurements of H+ and O-2 fluxes in Zostera marina and its physiological implications".PHYSIOLOGIA PLANTARUM 148.4(2013):582-589.

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