Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence | |
| Hao, Bin1,2; Mo, Zhao-Lan1,3; Xiao, Peng1; Pan, Hai-Jian1,2; Lan, Xin1,2; Li, Gui-Yang1,3; Mo, ZL | |
| 2013-03-01 | |
| 发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| ISSN | 0175-7598 |
| 卷号 | 97期号:6页码:2575-2585 |
| 文章类型 | Article |
| 摘要 | The sigma factor sigma(54) (RpoN) is an important regulator of bacterial response to environmental stresses. Here, we demonstrate the roles of RpoN in Vibrio anguillarum M3 by comparative investigation of physiological phenotypes and virulence of the wild-type, an rpoN mutant, and an rpoN complemented strain. Disruption of rpoN was found to decrease biofilm formation, production of exopolysaccharides, and production of the metalloproteases EmpA and PrtV. Injection experiments in fish showed that the M3 Delta rpoN mutant was attenuated in virulence when administrated either by intramuscular injection or by immersion challenge. Slower proliferation of the mutant in fish was also observed. Complementation of the mutant strain with rpoN restored some of the phenotypes to wild-type levels. RpoN was involved in regulation of some virulence-associated genes, as shown by real-time quantitative reverse PCR analysis. These results revealed a pleiotropic regulatory role of RpoN in biofilm formation, production of proteases and exopolysaccharides, and virulence in V. anguillarum M3.; The sigma factor sigma(54) (RpoN) is an important regulator of bacterial response to environmental stresses. Here, we demonstrate the roles of RpoN in Vibrio anguillarum M3 by comparative investigation of physiological phenotypes and virulence of the wild-type, an rpoN mutant, and an rpoN complemented strain. Disruption of rpoN was found to decrease biofilm formation, production of exopolysaccharides, and production of the metalloproteases EmpA and PrtV. Injection experiments in fish showed that the M3 Delta rpoN mutant was attenuated in virulence when administrated either by intramuscular injection or by immersion challenge. Slower proliferation of the mutant in fish was also observed. Complementation of the mutant strain with rpoN restored some of the phenotypes to wild-type levels. RpoN was involved in regulation of some virulence-associated genes, as shown by real-time quantitative reverse PCR analysis. These results revealed a pleiotropic regulatory role of RpoN in biofilm formation, production of proteases and exopolysaccharides, and virulence in V. anguillarum M3. |
| 关键词 | Vibrio Anguillarum Rpon (Sigma(54)) Biofilm Exopolysaccharides Metalloproteases Virulence |
| 学科领域 | Biotechnology & Applied Microbiology |
| DOI | 10.1007/s00253-012-4372-x |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000315650100027 |
| WOS关键词 | MEDIATED IRON UPTAKE ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; CHEMOTACTIC MOTILITY ; HEMOLYSIN GENE ; UPTAKE SYSTEM ; METALLOPROTEASE ; IDENTIFICATION ; EXPRESSION ; COLONIZATION |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16621 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Mo, ZL |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Hao, Bin,Mo, Zhao-Lan,Xiao, Peng,et al. Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(6):2575-2585. |
| APA | Hao, Bin.,Mo, Zhao-Lan.,Xiao, Peng.,Pan, Hai-Jian.,Lan, Xin.,...&Mo, ZL.(2013).Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(6),2575-2585. |
| MLA | Hao, Bin,et al."Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.6(2013):2575-2585. |
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