Preparation of D-glucosamine by hydrolysis of chitosan with chitosanase and beta-D-glucosaminidase

doi:10.1016/j.ijbiomac.2013.06.033

IOCAS-IR > 实验海洋生物学重点实验室

	Preparation of D-glucosamine by hydrolysis of chitosan with chitosanase and beta-D-glucosaminidase
	Sun, Yuying 1; Zhang, Jiquan 2; Wu, Shengjun 1; Wang, Shujun 1; Zhang, JQ
	2013-10-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	61 页码:160-163
文章类型	Article
摘要	Crude enzymes including chitosanase and beta-D-glucosaminidase were obtained by centrifugal separation from the fermentation broth of Microbacterium sp. OU01. Then the crude enzymes are used to hydrolyze chitosan for producing D-glucosamine (GlcN). The effects of temperature, pH, substrate concentration, the ratio of enzyme to chitosan, and hydrolysis time on the productivity of GlcN were discussed. The experimental result showed that the optimal conditions were temperature 50 degrees C, pH 5.8, substrate concentration 20 mg/mL, the optimum ratio of enzyme to chitosan 1.5 U/60 mg. Under above conditions, chitosan was completely hydrolyzed in 5 h. These results provide a scientific material for the optimization process of enzymatic production of GlcN. What is more, thin layer chromatography and high performance liquid chromatography were used to analyze hydrolytic product, which was proved to be GlcN. (C) 2013 Elsevier B.V. All rights reserved.; Crude enzymes including chitosanase and beta-D-glucosaminidase were obtained by centrifugal separation from the fermentation broth of Microbacterium sp. OU01. Then the crude enzymes are used to hydrolyze chitosan for producing D-glucosamine (GlcN). The effects of temperature, pH, substrate concentration, the ratio of enzyme to chitosan, and hydrolysis time on the productivity of GlcN were discussed. The experimental result showed that the optimal conditions were temperature 50 degrees C, pH 5.8, substrate concentration 20 mg/mL, the optimum ratio of enzyme to chitosan 1.5 U/60 mg. Under above conditions, chitosan was completely hydrolyzed in 5 h. These results provide a scientific material for the optimization process of enzymatic production of GlcN. What is more, thin layer chromatography and high performance liquid chromatography were used to analyze hydrolytic product, which was proved to be GlcN. (C) 2013 Elsevier B.V. All rights reserved.
关键词	Chitosanase Beta-d-glucosaminidase D-glucosamine
学科领域	Biochemistry & Molecular Biology
DOI	10.1016/j.ijbiomac.2013.06.033
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000326660700024
WOS关键词	ACETYL-D-GLUCOSAMINE ; PURIFICATION ; DEGRADATION ; CHITIN ; ACID
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16617
专题	实验海洋生物学重点实验室
通讯作者	Zhang, JQ
作者单位	1.Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yuying,Zhang, Jiquan,Wu, Shengjun,et al. Preparation of D-glucosamine by hydrolysis of chitosan with chitosanase and beta-D-glucosaminidase[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2013,61:160-163.
APA	Sun, Yuying,Zhang, Jiquan,Wu, Shengjun,Wang, Shujun,&Zhang, JQ.(2013).Preparation of D-glucosamine by hydrolysis of chitosan with chitosanase and beta-D-glucosaminidase.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,61,160-163.
MLA	Sun, Yuying,et al."Preparation of D-glucosamine by hydrolysis of chitosan with chitosanase and beta-D-glucosaminidase".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 61(2013):160-163.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Preparation of D-glu（563KB）			限制开放	CC BY-NC-SA	浏览