Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta)

doi:10.1007/s10811-012-9911-4

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	Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta)
	Wang, Shasha 1; Zhao, Fengyang 2; Wei, Xiaojiao 1; Lu, Bojun 1; Duan, Delin 3; Wang, Gaoge 1; Wang, GG
	2013-08-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	25 期号:4 页码:1215-1223
文章类型	Article
摘要	Flg22, which is the most conserved 22-amino acid peptide in the N-terminal part of flagellin, functions as an effective elicitor in higher plants. Marine algae and higher plants share some conserved characteristics on defense response pathways. Flg22-induced defense responses were investigated in female gametophytes of Saccharina japonica. Condensation of chloroplasts and thickened cell walls, as well as relatively stable structures of mitochondria and nucleus indicated that there were hypersensitive programmed cell death occurred after induction by flg22. By using luminol-dependent luminescence detection method, rapid release of H2O2 was detected in the induced female gametophytes and reached a peak concentration of about 46 mu M at 2 h. Reactive oxygen species production was also observed histologically using the fluorescent dye 2',7'-dichlorofluorescein diacetate, showing a consistent result with quantitative analysis of H2O2. Furthermore, results of antioxidant enzyme activities indicated that there was a trend in the order of catalase > superoxide dismutase > glutathione peroxidase. Finally, high level phenol content of cell-free extracts was found after flg22 induction. According to our results, flg22 could be an effective elicitor which could induce defense responses in female gametophytes of S. japonica.; Flg22, which is the most conserved 22-amino acid peptide in the N-terminal part of flagellin, functions as an effective elicitor in higher plants. Marine algae and higher plants share some conserved characteristics on defense response pathways. Flg22-induced defense responses were investigated in female gametophytes of Saccharina japonica. Condensation of chloroplasts and thickened cell walls, as well as relatively stable structures of mitochondria and nucleus indicated that there were hypersensitive programmed cell death occurred after induction by flg22. By using luminol-dependent luminescence detection method, rapid release of H2O2 was detected in the induced female gametophytes and reached a peak concentration of about 46 mu M at 2 h. Reactive oxygen species production was also observed histologically using the fluorescent dye 2',7'-dichlorofluorescein diacetate, showing a consistent result with quantitative analysis of H2O2. Furthermore, results of antioxidant enzyme activities indicated that there was a trend in the order of catalase > superoxide dismutase > glutathione peroxidase. Finally, high level phenol content of cell-free extracts was found after flg22 induction. According to our results, flg22 could be an effective elicitor which could induce defense responses in female gametophytes of S. japonica.
关键词	Defense Response Elicitor Female Gametophyte Flg22 Saccharina Japonica Phaeophyta
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10811-012-9911-4
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS类目	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS记录号	WOS:000321588800032
WOS关键词	PROGRAMMED CELL-DEATH ; GRACILARIA-CONFERTA RHODOPHYTA ; BACTERIAL ELICITOR FLAGELLIN ; INNATE IMMUNITY ; OXIDATIVE BURST ; ARABIDOPSIS-THALIANA ; PSEUDOMONAS-AVENAE ; LAMINARIA-DIGITATA ; HYDROGEN-PEROXIDE ; PERCEPTION
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16616
专题	实验海洋生物学重点实验室
通讯作者	Wang, GG
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Qingdao 39 Middle Sch, Qingdao 266003, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Shasha,Zhao, Fengyang,Wei, Xiaojiao,et al. Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2013,25(4):1215-1223.
APA	Wang, Shasha.,Zhao, Fengyang.,Wei, Xiaojiao.,Lu, Bojun.,Duan, Delin.,...&Wang, GG.(2013).Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta).JOURNAL OF APPLIED PHYCOLOGY,25(4),1215-1223.
MLA	Wang, Shasha,et al."Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta)".JOURNAL OF APPLIED PHYCOLOGY 25.4(2013):1215-1223.

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