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| Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: Gene structure, expression profile, and immunoregulatory property | |
| Chi, Heng; Xiao, Zhi-zhong; Sun, Li; Sun, L | |
| 2013-09-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| 卷号 | 35期号:3页码:972-978 |
| 文章类型 | Article |
| 摘要 | Nuclear factor 45 (NF45) is a component of the protein complex called nuclear factor of activated T-cells (NFAT), which in mammals regulates interleukin (IL)-2 expression. To date very little is known about fish NF45. In this study, we identified a NF45 (named CsNF45) from half smooth tongue sole Cynoglossus semilaevis and examined its gene organization, expression profile, and regulatory function. We found that CsNF45 is composed of 387 residues and shares 90.3%-97.9% overall sequence identities with the NF45 of human and teleosts. Genetic analysis showed that the genomic sequence of the ORF region of CsNF45 consists of 14 exons and 13 introns. Constitutive expression of CsNF45 occurred in multiple tissues including gill, muscle, brain, heart, liver, head kidney, spleen, and gut. Experimental infection with viral and bacterial pathogens upregulated the expression of CsNF45 in head kidney and spleen in a time-dependent manner. Transient transfection analysis showed that CsNF45 was localized in the nucleus and able to stimulate the activity of mouse IL-2 promoter. These results indicate that CsNF45 possesses immunoregulatory property and is possibly involved in host immune defense against bacterial and viral infection. (C) 2013 Elsevier Ltd. All rights reserved.; Nuclear factor 45 (NF45) is a component of the protein complex called nuclear factor of activated T-cells (NFAT), which in mammals regulates interleukin (IL)-2 expression. To date very little is known about fish NF45. In this study, we identified a NF45 (named CsNF45) from half smooth tongue sole Cynoglossus semilaevis and examined its gene organization, expression profile, and regulatory function. We found that CsNF45 is composed of 387 residues and shares 90.3%-97.9% overall sequence identities with the NF45 of human and teleosts. Genetic analysis showed that the genomic sequence of the ORF region of CsNF45 consists of 14 exons and 13 introns. Constitutive expression of CsNF45 occurred in multiple tissues including gill, muscle, brain, heart, liver, head kidney, spleen, and gut. Experimental infection with viral and bacterial pathogens upregulated the expression of CsNF45 in head kidney and spleen in a time-dependent manner. Transient transfection analysis showed that CsNF45 was localized in the nucleus and able to stimulate the activity of mouse IL-2 promoter. These results indicate that CsNF45 possesses immunoregulatory property and is possibly involved in host immune defense against bacterial and viral infection. (C) 2013 Elsevier Ltd. All rights reserved. |
| 关键词 | Nuclear Factor 45 Interleukin-2 Enhancer Binding Factor 2 Cynoglossus Semilaevis |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2013.07.016 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000324511700042 |
| WOS关键词 | RNA-BINDING PROTEINS ; ACTIVATED T-CELLS ; HUMAN IL-2 GENE ; TRANSLATIONAL CONTROL ; INTERLEUKIN-2 GENE ; CYCLOSPORINE-A ; IN-VIVO ; RGG BOX ; NF90 ; PROMOTER |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16611 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, L |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Chi, Heng,Xiao, Zhi-zhong,Sun, Li,et al. Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: Gene structure, expression profile, and immunoregulatory property[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):972-978. |
| APA | Chi, Heng,Xiao, Zhi-zhong,Sun, Li,&Sun, L.(2013).Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: Gene structure, expression profile, and immunoregulatory property.FISH & SHELLFISH IMMUNOLOGY,35(3),972-978. |
| MLA | Chi, Heng,et al."Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: Gene structure, expression profile, and immunoregulatory property".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):972-978. |
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