Mere15, a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs

doi:10.3109/13880209.2012.715169

IOCAS-IR > 实验海洋生物学重点实验室

	Mere15, a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs
	Wang, Hui1,3 ; Wei, Jianteng 1,3; Wu, Ning 1; Liu, Ming 1; Wang, Cuicui 3; Zhang, Yuyan 1; Wang, Fengxia 1; Liu, Haizhou 1; Lin, Xiukun 1,2; Lin, XK
	2013-02-01
发表期刊	PHARMACEUTICAL BIOLOGY
ISSN	1388-0209
卷号	51 期号:2 页码:145-151
文章类型	Article
摘要	Context: Mere15 is a novel antitumor polypeptide purified from Meretrix meretrix Linn. (Veneridae). Previous studies have shown that the polypeptide induced cell death via intrinsic mitochondrial pathway.; Context: Mere15 is a novel antitumor polypeptide purified from Meretrix meretrix Linn. (Veneridae). Previous studies have shown that the polypeptide induced cell death via intrinsic mitochondrial pathway. Objective: In the present study, the effects of Mere15 on cell adhesion, migration, invasion, as well as secretion and expression of matrix metalloproteinases (MMPs) were studied in human lung adenocarcinoma A549 cells. Materials and methods: The effect of Mere15 on cell adhesion, migration and invasion were studied by cell adhesion and transwell assay. The expression of MMPs was determined by gelatin zymography and RT-PCR analysis. Results: The ability of cell adhesion was decreased by 86.74% at the concentration of 12.0 mu g/mL of Mere15. And the migration and invasion of A549 cells were decreased with the inhibition ratio of, 69.22 and 53.84% when treated with 15.0 mu g/mL of Mere15. Further study also revealed that treatment of the cancer cells with Mere15 (15.0 mu g/mL), the secretion of MMP-2 and MMP-9 were down-regulated with the inhibition ratio of 72.00 and 93.24% and the inhibition rate of mRNA expression of MMP-2 and MMP-9 was 57.54 and 91.22%, respectively. Discussion and conclusion: The study demonstrates that Mere15 inhibits tumor growth via both pro-apoptotic and antimetastasis pathways, and the polypeptide has potential to be developed as a multi-target therapeutic agent for the treatment of human lung cancer.
关键词	Anticancer Cancer Metastasis Transwell Assay Gelatin Zymography Rt-pcr
学科领域	Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
DOI	10.3109/13880209.2012.715169
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
WOS类目	Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
WOS记录号	WOS:000313676300002
WOS关键词	EXPRESSION ; PROLIFERATION ; GROWTH ; ACID
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16603
专题	实验海洋生物学重点实验室
通讯作者	Lin, XK
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Capital Med Univ, Dept Pharmacol, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Hui,Wei, Jianteng,Wu, Ning,et al. Mere15, a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs[J]. PHARMACEUTICAL BIOLOGY,2013,51(2):145-151.
APA	Wang, Hui.,Wei, Jianteng.,Wu, Ning.,Liu, Ming.,Wang, Cuicui.,...&Lin, XK.(2013).Mere15, a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs.PHARMACEUTICAL BIOLOGY,51(2),145-151.
MLA	Wang, Hui,et al."Mere15, a novel polypeptide from Meretrix meretrix, inhibits adhesion, migration and invasion of human lung cancer A549 cells via down-regulating MMPs".PHARMACEUTICAL BIOLOGY 51.2(2013):145-151.

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