Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China | |
Liu, Haizhou1; Xiao, Lin1,2; Wei, Jianteng1; Schmitz, John C.3; Liu, Ming1; Wang, Cuicui1; Cheng, Linyou1; Wu, Ning1; Chen, Lei1 ; Zhang, Yuyan4; Lin, Xiukun1,5; Lin, XK
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| 2013-07-01 | |
| 发表期刊 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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| ISSN | 0959-3993 |
| 卷号 | 29期号:7页码:1271-1278 |
| 文章类型 | Article |
| 摘要 | A moderately halophilic actinomycetes strain, designated as WH26, was isolated from Weihai Solar Saltern in China. The identification of the strain WH26 was performed by its morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on 16S rRNA sequence comparison. The results showed that the nucleotide sequence of the 16S rRNA gene (1,677 bp) of the strain WH26 exhibited close similarity (97-99 %) with other Streptomyces 16S rRNA genes and the strain WH26 was identified to belong to the genus Streptomyces. An ethyl acetate extraction of Streptomyces sp. nov. WH26 demonstrated significant cellular toxicity. Two compounds, 8-O-methyltetrangulol and naphthomycin A were isolated from the extract via silica gel column chromatography and HPLC. These two compounds showed potent cytotoxic activity against several human tumor cell lines including A549, HeLa, BEL-7402 and HT-29. The present studies suggest that moderately halophilic actinomycetes may be a novel biological source for the discovery of anticancer agents.; A moderately halophilic actinomycetes strain, designated as WH26, was isolated from Weihai Solar Saltern in China. The identification of the strain WH26 was performed by its morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on 16S rRNA sequence comparison. The results showed that the nucleotide sequence of the 16S rRNA gene (1,677 bp) of the strain WH26 exhibited close similarity (97-99 %) with other Streptomyces 16S rRNA genes and the strain WH26 was identified to belong to the genus Streptomyces. An ethyl acetate extraction of Streptomyces sp. nov. WH26 demonstrated significant cellular toxicity. Two compounds, 8-O-methyltetrangulol and naphthomycin A were isolated from the extract via silica gel column chromatography and HPLC. These two compounds showed potent cytotoxic activity against several human tumor cell lines including A549, HeLa, BEL-7402 and HT-29. The present studies suggest that moderately halophilic actinomycetes may be a novel biological source for the discovery of anticancer agents. |
| 关键词 | Moderately Halophilic Actinomycetes Streptomyces 8-o-methyltetrangulol Naphthomycin a |
| 学科领域 | Biotechnology & Applied Microbiology |
| DOI | 10.1007/s11274-013-1290-8 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000321976800015 |
| WOS关键词 | HALOPHILIC ACTINOMYCETE ; STRUCTURE ELUCIDATION ; BIOLOGICAL-ACTIVITY ; SEQUENCE ALIGNMENT ; NAPHTHOMYCIN ; ANSAMYCIN ; SEDIMENTS ; GENE ; TETRANGOMYCIN ; TETRANGULOL |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16591 |
| 专题 | 实验海洋生物学重点实验室 海洋生态与环境科学重点实验室 |
| 通讯作者 | Lin, XK |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Shandong, Peoples R China 3.Univ Pittsburgh, Dept Med, Pittsburgh, PA 15213 USA 4.Qingdao Univ Sci & Technol, Sch Chem Engn, Qingdao 266042, Shandong, Peoples R China 5.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Haizhou,Xiao, Lin,Wei, Jianteng,et al. Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(7):1271-1278. |
| APA | Liu, Haizhou.,Xiao, Lin.,Wei, Jianteng.,Schmitz, John C..,Liu, Ming.,...&Lin, XK.(2013).Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(7),1271-1278. |
| MLA | Liu, Haizhou,et al."Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.7(2013):1271-1278. |
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