Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification of a tyrosinase gene potentially involved in early larval shell biogenesis of the Pacific oyster Crassostrea gigas | |
Huan, Pin1 ; Liu, Gang2; Wang, Hongxia1; Liu, Baozhong1; Liu, BZ
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| 2013-11-01 | |
| 发表期刊 | DEVELOPMENT GENES AND EVOLUTION
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| ISSN | 0949-944X |
| 卷号 | 223期号:6页码:389-394 |
| 文章类型 | Article |
| 摘要 | The larval shell emerges early in embryogenesis of mollusks, but the detailed mechanisms of its biogenesis remain to be determined. In this study, we cloned a tyrosinase gene (cgi-tyr1) that potentially functioned in larval shell biogenesis from the Pacific oyster Crassostrea gigas, a worldwide bivalve species. Sequence analysis of cgi-tyr1 revealed that it had typical copper-binding domains and a signal peptide. Through whole mount in situ hybridization and an electron scanning microscopic observation, we detected the expression of cgi-tyr1 firstly in the saddle-shaped shell field in trochophores, indicating that cgi-tyr1 might participate in the biogenesis of the initial non-calcified shell of trochophores. In the following development to early D-veliger, cells in the central region of shell field exhibited no detectable cgi-tyr1 expression, and cgi-tyr1 expression was sustained only in the edge of the shell field and the hinge region, indicating that cgi-tyr1 might function fundamentally in shell growth from trochophore to early D-veliger. Unexpectedly, cgi-tyr1 expression was not detected after the D-veliger stage. This indicated that other molecules might function in later shell development. Our results suggested a role for a tyrosinase gene that specifically functioned in the initial phase of the larval shell biogenesis of C. gigas. This work would shed light on future studies on larval shell development and might be helpful to understand how the molluscan shell emerged during evolution.; The larval shell emerges early in embryogenesis of mollusks, but the detailed mechanisms of its biogenesis remain to be determined. In this study, we cloned a tyrosinase gene (cgi-tyr1) that potentially functioned in larval shell biogenesis from the Pacific oyster Crassostrea gigas, a worldwide bivalve species. Sequence analysis of cgi-tyr1 revealed that it had typical copper-binding domains and a signal peptide. Through whole mount in situ hybridization and an electron scanning microscopic observation, we detected the expression of cgi-tyr1 firstly in the saddle-shaped shell field in trochophores, indicating that cgi-tyr1 might participate in the biogenesis of the initial non-calcified shell of trochophores. In the following development to early D-veliger, cells in the central region of shell field exhibited no detectable cgi-tyr1 expression, and cgi-tyr1 expression was sustained only in the edge of the shell field and the hinge region, indicating that cgi-tyr1 might function fundamentally in shell growth from trochophore to early D-veliger. Unexpectedly, cgi-tyr1 expression was not detected after the D-veliger stage. This indicated that other molecules might function in later shell development. Our results suggested a role for a tyrosinase gene that specifically functioned in the initial phase of the larval shell biogenesis of C. gigas. This work would shed light on future studies on larval shell development and might be helpful to understand how the molluscan shell emerged during evolution. |
| 关键词 | Bivalve Development Periostracum Whole Mount In Situ Hybridization |
| 学科领域 | Cell Biology ; Evolutionary Biology ; Developmental Biology |
| DOI | 10.1007/s00427-013-0450-z |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Cell Biology ; Evolutionary Biology ; Developmental Biology |
| WOS类目 | Cell Biology ; Evolutionary Biology ; Developmental Biology |
| WOS记录号 | WOS:000325810600005 |
| WOS关键词 | PROTEINS ; BIVALVIA ; PERIOSTRACUM ; EXPRESSION |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16589 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, BZ |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huan, Pin,Liu, Gang,Wang, Hongxia,et al. Identification of a tyrosinase gene potentially involved in early larval shell biogenesis of the Pacific oyster Crassostrea gigas[J]. DEVELOPMENT GENES AND EVOLUTION,2013,223(6):389-394. |
| APA | Huan, Pin,Liu, Gang,Wang, Hongxia,Liu, Baozhong,&Liu, BZ.(2013).Identification of a tyrosinase gene potentially involved in early larval shell biogenesis of the Pacific oyster Crassostrea gigas.DEVELOPMENT GENES AND EVOLUTION,223(6),389-394. |
| MLA | Huan, Pin,et al."Identification of a tyrosinase gene potentially involved in early larval shell biogenesis of the Pacific oyster Crassostrea gigas".DEVELOPMENT GENES AND EVOLUTION 223.6(2013):389-394. |
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