Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment | |
| Liu, Rui1; Qiu, Limei1; Yu, Zuoan3; Zi, Jun1; Yue, Feng1,2; Wang, Lingling1; Zhang, Huan1; Teng, Weiming3; Liu, Xiangfeng3; Song, Linsheng1; Song, LS | |
| 2013-10-01 | |
| 发表期刊 | JOURNAL OF INVERTEBRATE PATHOLOGY
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| ISSN | 0022-2011 |
| 卷号 | 114期号:2页码:144-150 |
| 文章类型 | Article |
| 摘要 | A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 strains), Pseudomonas (2 strains) and Shewanella (1 strain) by 16S rDNA sequencing. In the Vibrio group, Vibrio splendidus was the predominant species, consisting of 43 strains. A strain of V. splendidus, named JZ6, was identified as a potential pathogen because it possessed two important virulence factors, Vsm and OmpU. Stain JZ6 was cold-adapted, and could surviveand exhibit haemolytic activity from 4 degrees C to 32 degrees C, with the highest activity observed at 10 degrees C. The pathogenicity of JZ6 was further confirmed by injection and immersion challenges to scallops at 10 degrees C. The cumulative mortality rates in the two, experiments were 80% and 45%, respectively, which were significantly higher (P < 0.05) than those of control and blank groups. The disorganisation of muscle fibres in challenged scallops was observed to be histopathologically identical to that in moribund Yesso scallop collected from the same farm. The results indicate that V. splendidus is the predominantly culturable bacteria from the lesions of diseased Yesso scallop, and JZ6 probably is the pathogenic agent to Yesso scallop in this low temperature environment. (C) 2013 Elsevier Inc. All rights reserved.; A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 strains), Pseudomonas (2 strains) and Shewanella (1 strain) by 16S rDNA sequencing. In the Vibrio group, Vibrio splendidus was the predominant species, consisting of 43 strains. A strain of V. splendidus, named JZ6, was identified as a potential pathogen because it possessed two important virulence factors, Vsm and OmpU. Stain JZ6 was cold-adapted, and could surviveand exhibit haemolytic activity from 4 degrees C to 32 degrees C, with the highest activity observed at 10 degrees C. The pathogenicity of JZ6 was further confirmed by injection and immersion challenges to scallops at 10 degrees C. The cumulative mortality rates in the two, experiments were 80% and 45%, respectively, which were significantly higher (P < 0.05) than those of control and blank groups. The disorganisation of muscle fibres in challenged scallops was observed to be histopathologically identical to that in moribund Yesso scallop collected from the same farm. The results indicate that V. splendidus is the predominantly culturable bacteria from the lesions of diseased Yesso scallop, and JZ6 probably is the pathogenic agent to Yesso scallop in this low temperature environment. (C) 2013 Elsevier Inc. All rights reserved. |
| 关键词 | Pathogencity Bacterial Infection Bivalve Disease Lesion Low Temperature |
| 学科领域 | Zoology |
| DOI | 10.1016/j.jip.2013.07.005 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Zoology |
| WOS类目 | Zoology |
| WOS记录号 | WOS:000324790400006 |
| WOS关键词 | BROWN RING DISEASE ; CLAM RUDITAPES-PHILIPPINARUM ; CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; SHELLFISH ; BACTERIA ; TAPETIS ; ALGINOLYTICUS ; MORTALITIES ; RESPONSES |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16586 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Song, LS |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Liaoning Ocean & Fisheries Sci Res Inst, Liaoning Key Lab Marine Fisheries Mol Biol, Dalian 116023, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, Rui,Qiu, Limei,Yu, Zuoan,et al. Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2013,114(2):144-150. |
| APA | Liu, Rui.,Qiu, Limei.,Yu, Zuoan.,Zi, Jun.,Yue, Feng.,...&Song, LS.(2013).Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment.JOURNAL OF INVERTEBRATE PATHOLOGY,114(2),144-150. |
| MLA | Liu, Rui,et al."Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment".JOURNAL OF INVERTEBRATE PATHOLOGY 114.2(2013):144-150. |
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