Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei

doi:10.1186/1471-2148-13-165

IOCAS-IR > 实验海洋生物学重点实验室

	Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei
	Yuan, Jian-Bo 1,2; Zhang, Xiao-Jun 1; Liu, Cheng-Zhang 1; Wei, Jian-Kai1,2 ; Li, Fu-Hua 1; Xiang, Jian-Hai1 ; Zhang, XJ
	2013-08-06
发表期刊	BMC EVOLUTIONARY BIOLOGY
ISSN	1471-2148
卷号	13
文章类型	Article
摘要	Background: In recent years, as the development of next-generation sequencing technology, a growing number of genes have been reported as being horizontally transferred from prokaryotes to eukaryotes, most of them involving arthropods. As a member of the phylum Arthropoda, the Pacific white shrimp Litopenaeus vannamei has to adapt to the complex water environments with various symbiotic or parasitic microorganisms, which provide a platform for horizontal gene transfer (HGT).; Background: In recent years, as the development of next-generation sequencing technology, a growing number of genes have been reported as being horizontally transferred from prokaryotes to eukaryotes, most of them involving arthropods. As a member of the phylum Arthropoda, the Pacific white shrimp Litopenaeus vannamei has to adapt to the complex water environments with various symbiotic or parasitic microorganisms, which provide a platform for horizontal gene transfer (HGT). Results: In this study, we analyzed the genome-wide HGT events in L. vannamei. Through homology search and phylogenetic analysis, followed by experimental PCR confirmation, 14 genes with HGT event were identified: 12 of them were transferred from bacteria and two from fungi. Structure analysis of these genes showed that the introns of the two fungi-originated genes were substituted by shrimp DNA fragment, two genes transferred from bacteria had shrimp specific introns inserted in them. Furthermore, around other three bacteria-originated genes, there were three large DNA segments inserted into the shrimp genome. One segment was a transposon that fully transferred, and the other two segments contained only coding regions of bacteria. Functional prediction of these 14 genes showed that 6 of them might be related to energy metabolism, and 4 others related to defense of the organism. Conclusions: HGT events from bacteria or fungi were happened in the genome of L. vannamei, and these horizontally transferred genes can be transcribed in shrimp. This is the first time to report the existence of horizontally transferred genes in shrimp. Importantly, most of these genes are exposed to a negative selection pressure and appeared to be functional.
关键词	Horizontal Gene Transfer Litopenaeus Vannamei Shrimp Bacteria Fungi
学科领域	Evolutionary Biology ; Genetics & Heredity
DOI	10.1186/1471-2148-13-165
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Evolutionary Biology ; Genetics & Heredity
WOS类目	Evolutionary Biology ; Genetics & Heredity
WOS记录号	WOS:000323026300001
WOS关键词	ESCHERICHIA-COLI ; EXPRESSION ANALYSIS ; DIFFERENTIAL EXPRESSION ; O-METHYLTRANSFERASE ; DNA-REPLICATION ; EVOLUTION ; BACTERIA ; PREDICTION ; TRANSCRIPTOME ; CHROMOSOME
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16584
专题	实验海洋生物学重点实验室
通讯作者	Zhang, XJ
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yuan, Jian-Bo,Zhang, Xiao-Jun,Liu, Cheng-Zhang,et al. Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei[J]. BMC EVOLUTIONARY BIOLOGY,2013,13.
APA	Yuan, Jian-Bo.,Zhang, Xiao-Jun.,Liu, Cheng-Zhang.,Wei, Jian-Kai.,Li, Fu-Hua.,...&Zhang, XJ.(2013).Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei.BMC EVOLUTIONARY BIOLOGY,13.
MLA	Yuan, Jian-Bo,et al."Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei".BMC EVOLUTIONARY BIOLOGY 13(2013).

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