Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Genetic similarity analysis within Pyropia yezoensis blades developed from both conchospores and blade archeospores using AFLP | |
| He, Linwen1,2; Zhu, Jianyi3; Lu, Qinqin4; Niu, Jianfeng1; Zhang, Baoyu1; Lin, Apeng1; Wang, Guangce1 | |
| 2013-06-01 | |
| 发表期刊 | JOURNAL OF PHYCOLOGY
 |
| ISSN | 0022-3646 |
| 卷号 | 49期号:3页码:517-522 |
| 摘要 | Pyropia yezoensis (Ueda) M. S. Hwang et H. G. Choi (previously called Porphyra yezoensis) is an economically important alga. The blades generated from conchospores are genetic chimeras, which are not suitable for genetic similarity analysis. In this study, two types of blades from a single filament of P.yezoensis sporophyte filament were obtained. One type, ConB, consisted of 40 blades that had germinated from conchospores. The other type, ArcB, consisted of 88 blades that had germinated from archeospores released from ConB. Both of them were analyzed by amplified fragment length polymorphism. The low genetic similarity levels for both conchospore-germinated and archeospore-germinated blades demonstrated that the conchcelis we used was cross-fertilized. Furthermore, a higher polymorphic loci ratio (98.6%) was detected in ArcB than in ConB (80.7%), and the average genetic similarity of ArcB (average 0.61) was lower than that of ConB (average 0.71). These differences indicated that genetic analysis using ArcB gives more accurate results. |
| 关键词 | AFLP archeospore conchospore genetic similarity Pyropia yezoensis |
| DOI | 10.1111/jpy.12058 |
| 语种 | 英语 |
| 资助项目 | 863 project[2012AA100806]; 973 project[2011CB411908]; National Natural Science Foundation of China[40906074]; nonprofit research project for the State Oceanic Administration[201105023-2]; nonprofit research project for the State Oceanic Administration[201105023-7]; Ministry of Agriculture of China[200903030] |
| WOS研究方向 | Plant Sciences ; Marine & Freshwater Biology |
| WOS类目 | Plant Sciences ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000319874900009 |
| 出版者 | WILEY-BLACKWELL |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16577 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci IOCAS, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Changshu Inst Technol, Dept Biol & Food Engn, Changshu 215500, Peoples R China 4.Jiangsu Inst Oceanol & Marine Fisheries, State Germplasm Bank Porphyra, Nantong 226007, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | He, Linwen,Zhu, Jianyi,Lu, Qinqin,et al. Genetic similarity analysis within Pyropia yezoensis blades developed from both conchospores and blade archeospores using AFLP[J]. JOURNAL OF PHYCOLOGY,2013,49(3):517-522. |
| APA | He, Linwen.,Zhu, Jianyi.,Lu, Qinqin.,Niu, Jianfeng.,Zhang, Baoyu.,...&Wang, Guangce.(2013).Genetic similarity analysis within Pyropia yezoensis blades developed from both conchospores and blade archeospores using AFLP.JOURNAL OF PHYCOLOGY,49(3),517-522. |
| MLA | He, Linwen,et al."Genetic similarity analysis within Pyropia yezoensis blades developed from both conchospores and blade archeospores using AFLP".JOURNAL OF PHYCOLOGY 49.3(2013):517-522. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Genetic similarity a（192KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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