Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria | |
Sun Yumiao1,2; Li Fuhua1; Chi Yanhong1,2; Xiang Jianhai1 ; Xiang, JH
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| 2013-02-01 | |
| 发表期刊 | ACTA OCEANOLOGICA SINICA
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| ISSN | 0253-505X |
| 卷号 | 32期号:2页码:52-58 |
| 文章类型 | Article |
| 摘要 | Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB couldmake VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67 +/- 0.08, compared with 0.41 +/- 0.09 of bacteria without VP28 (P < 0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.; Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB couldmake VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67 +/- 0.08, compared with 0.41 +/- 0.09 of bacteria without VP28 (P < 0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV. |
| 关键词 | Wssv Vp28 Exopalamon Carincauda Live Bacterial deLiver |
| 学科领域 | Oceanography |
| DOI | 10.1007/s13131-013-0261-0 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Oceanography |
| WOS类目 | Oceanography |
| WOS记录号 | WOS:000314699900007 |
| WOS关键词 | WHITE-SPOT-SYNDROME ; SYNDROME-VIRUS WSSV ; ENVELOPE PROTEIN VP28 ; PENAEUS-MONODON ; PROCAMBARUS-CLARKII ; PROTECTIVE IMMUNITY ; VACCINATION TRIALS ; ORAL VACCINATION ; SUBUNIT VACCINE ; GENOME SEQUENCE |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16564 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Xiang, JH |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Sun Yumiao,Li Fuhua,Chi Yanhong,et al. Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria[J]. ACTA OCEANOLOGICA SINICA,2013,32(2):52-58. |
| APA | Sun Yumiao,Li Fuhua,Chi Yanhong,Xiang Jianhai,&Xiang, JH.(2013).Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria.ACTA OCEANOLOGICA SINICA,32(2),52-58. |
| MLA | Sun Yumiao,et al."Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria".ACTA OCEANOLOGICA SINICA 32.2(2013):52-58. |
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| Enhanced resistance （239KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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