Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Cloning of TPS gene from eelgrass species Zostera marina and its functional identification by genetic transformation in rice | |
Zhao, Feng1,3; Li, Qiuying2,5 ; Weng, Manli3; Wang, Xiuliang2; Guo, Baotai4; Wang, Li3; Wang, Wei3; Duan, Delin2; Wang, Bin3; Duan, DL
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| 2013-12-01 | |
| 发表期刊 | GENE
 |
| ISSN | 0378-1119 |
| 卷号 | 531期号:2页码:205-211 |
| 文章类型 | Article |
| 摘要 | The full-length cDNA sequence (2613 bp) of the trehalose-6-phosphate synthase (ITS) gene of eelgrass Zostera marina (ZmTPS) was identified and cloned. Z. marina is a kind of seed-plant growing in sea water during its whole life history. The open reading frame (ORF) region of ZmTPS gene encodes a protein of 870 amino acid residues and a stop codon. The corresponding genomic DNA sequence is 3770 bp in length, which contains 3 exons and 2 introns. The ZmTPS gene was transformed into rice variety ZH11 via Agrobacterium-mediated transformation method. After antibiotic screening, molecular characterization, salt-tolerance and trehalose content determinations, two transgenic lines resistant to 150 mM NaCL solutions were screened. Our study results indicated that the ZmTPS gene was integrated into the genomic DNA of the two transgenic rice lines and could be expressed well. Moreover, the detection of the transformed ZmTPS gene in the progenies of the two transgenic lines was performed from T-1 to T-4 generations; and results suggested that the transformed ZmTPS gene can be transmitted from parent to the progeny in transgenic rice. (C) 2013 Elsevier B.V. All rights reserved.; The full-length cDNA sequence (2613 bp) of the trehalose-6-phosphate synthase (ITS) gene of eelgrass Zostera marina (ZmTPS) was identified and cloned. Z. marina is a kind of seed-plant growing in sea water during its whole life history. The open reading frame (ORF) region of ZmTPS gene encodes a protein of 870 amino acid residues and a stop codon. The corresponding genomic DNA sequence is 3770 bp in length, which contains 3 exons and 2 introns. The ZmTPS gene was transformed into rice variety ZH11 via Agrobacterium-mediated transformation method. After antibiotic screening, molecular characterization, salt-tolerance and trehalose content determinations, two transgenic lines resistant to 150 mM NaCL solutions were screened. Our study results indicated that the ZmTPS gene was integrated into the genomic DNA of the two transgenic rice lines and could be expressed well. Moreover, the detection of the transformed ZmTPS gene in the progenies of the two transgenic lines was performed from T-1 to T-4 generations; and results suggested that the transformed ZmTPS gene can be transmitted from parent to the progeny in transgenic rice. (C) 2013 Elsevier B.V. All rights reserved. |
| 关键词 | Agrobacterium-mediated Transformation Zostera Marina Salt-tolerance Transgenic Rice Trehalose-6-phosphate Synthase (Tps) |
| 学科领域 | Genetics & Heredity |
| DOI | 10.1016/j.gene.2013.09.016 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| WOS记录号 | WOS:000326846800012 |
| WOS关键词 | DROUGHT TOLERANCE ; TREHALOSE-6-PHOSPHATE SYNTHASE ; TREHALOSE ACCUMULATION ; TRANSGENIC RICE ; BIFUNCTIONAL FUSION ; PLANTS ; EXPRESSION ; TOBACCO ; INCREASES ; STRESS |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16545 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Duan, DL |
| 作者单位 | 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 4.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Feng,Li, Qiuying,Weng, Manli,et al. Cloning of TPS gene from eelgrass species Zostera marina and its functional identification by genetic transformation in rice[J]. GENE,2013,531(2):205-211. |
| APA | Zhao, Feng.,Li, Qiuying.,Weng, Manli.,Wang, Xiuliang.,Guo, Baotai.,...&Duan, DL.(2013).Cloning of TPS gene from eelgrass species Zostera marina and its functional identification by genetic transformation in rice.GENE,531(2),205-211. |
| MLA | Zhao, Feng,et al."Cloning of TPS gene from eelgrass species Zostera marina and its functional identification by genetic transformation in rice".GENE 531.2(2013):205-211. |
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| Cloning of TPS gene （1461KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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