Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China | |
| Xiao, Lin1,2,3; Liu, Haizhou1; Wu, Ning1; Liu, Ming1; Wei, Jianteng1; Zhang, Yuyan1; Lin, Xiukun1; Lin, XK | |
| 2013 | |
| 发表期刊 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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| ISSN | 0959-3993 |
| 卷号 | 29期号:1页码:11-17 |
| 文章类型 | Article |
| 摘要 | A moderately halophilic fungus F1 was isolated from a marine solar saltern in Weihai, China. The identification of the fungus F1 was performed by the morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on ITS (internal transcribed spacer)-5.8S rDNA region sequence comparison. The strain was identified as belonging to the genus Aspergillus and designated as Aspergillus sp. nov. F1. Furthermore, Aspergillus sp. nov. F1 grew well in 3-15 % (w/v) NaCl, and with increasing of salinity, the generation of secondary metabolites with cytotoxicity was also augmented. Three compounds with cytotoxicity were isolated from the ethyl acetate extract of the whole broth and mycelia of Aspergillus sp. nov. F1, and identified as ergosterol, rosellichalasin and cytochalasin E, respectively. Especially, ergosterol showed high potent cytotoxic activity to human colon cancer cell line RKO with IC50 of 3.3 +/- A 0.5 mu M. Considering the high cytochalasin production and the simple and economical fermentation of Aspergillus sp. nov. F1, the strain could be used as potential strain for large scale production of the cytochalasin E and rosellichalasin.; A moderately halophilic fungus F1 was isolated from a marine solar saltern in Weihai, China. The identification of the fungus F1 was performed by the morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on ITS (internal transcribed spacer)-5.8S rDNA region sequence comparison. The strain was identified as belonging to the genus Aspergillus and designated as Aspergillus sp. nov. F1. Furthermore, Aspergillus sp. nov. F1 grew well in 3-15 % (w/v) NaCl, and with increasing of salinity, the generation of secondary metabolites with cytotoxicity was also augmented. Three compounds with cytotoxicity were isolated from the ethyl acetate extract of the whole broth and mycelia of Aspergillus sp. nov. F1, and identified as ergosterol, rosellichalasin and cytochalasin E, respectively. Especially, ergosterol showed high potent cytotoxic activity to human colon cancer cell line RKO with IC50 of 3.3 +/- A 0.5 mu M. Considering the high cytochalasin production and the simple and economical fermentation of Aspergillus sp. nov. F1, the strain could be used as potential strain for large scale production of the cytochalasin E and rosellichalasin. |
| 关键词 | Moderately Halophilic Fungus Aspergillus Cytotoxic Activity Cytochalasin |
| 学科领域 | Biotechnology & Applied Microbiology |
| DOI | 10.1007/s11274-012-1152-9 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000312075300002 |
| WOS关键词 | SEQUENCE ALIGNMENT ; METABOLITE ; EXTRACTS ; BACTERIA ; HALOCINS ; GROWTH ; ACTIN ; CELLS |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16539 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Lin, XK |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Xiao, Lin,Liu, Haizhou,Wu, Ning,et al. Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(1):11-17. |
| APA | Xiao, Lin.,Liu, Haizhou.,Wu, Ning.,Liu, Ming.,Wei, Jianteng.,...&Lin, XK.(2013).Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(1),11-17. |
| MLA | Xiao, Lin,et al."Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.1(2013):11-17. |
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