High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution

doi:10.3389/fgene.2019.01340

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	High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution
	Tang, Boping 1; Wang, Zhongkai 2; Liu, Qiuning 1; Zhang, Huabin 1; Jiang, Senhao 1; Li, Xinzheng 3; Wang, Zhengfei 1; Sun, Yue 1; Sha, Zhongli3 ; Jiang, Hui 4,5; Wu, Xugan 6; Ren, Yandong 2; Li, Haorong 2; Xuan, Fujun 1; Ge, Baoming 1; Jiang, Wei3 ; She, Shusheng 7; Sun, Hongying 8; Qiu, Qiang 2; Wang, Wen 2; Wang, Qun 9; Qiu, Gaofeng 6; Zhang, Daizhen 1; Li, Yongxin 2
	2020-01-17
发表期刊	FRONTIERS IN GENETICS
卷号	10 页码:9
通讯作者	Wang, Qun([email protected]) ; Qiu, Gaofeng([email protected]) ; Zhang, Daizhen([email protected]) ; Li, Yongxin([email protected])
摘要	As an important freshwater aquaculture species in China, the Chinese mitten crab (Eriocheir japonica sinensis) has high economic and nutritional value. However, limited genomic information is currently available for studying its basic development and genetic diversity. Here, we performed whole-genome sequencing on Oxford Nanopore Technologies Limited's platform using promethION. The assembled size of E. j.sinensis genome was approximately 1.27 Gb, which is close to the estimated size (1.19 Gb). Furthermore, based on assessment using Benchmarking Universal Single-Copy Orthologs (BUSCO) (Simao et al., 2015), 94.00% of the expected eukaryotic genes were completely present in the genome assembly. In addition, repetitive sequences accounted for similar to 61.42% of the assembled genome, and 22,619 protein-coding genes were annotated. Comparative genomics analysis demonstrated that the Chinese mitten crab diverged from Penaeus vannamei similar to 373.6 million years ago, with a faster evolution rate than shrimp. We anticipate that the annotated high-quality genome of E. j. sinensis will promote research on its basic development and evolution and make substantial contributions to comparative genomic analyses of crustaceans.
关键词	Eriocheir japonica sinensis evolution nanopore crab genome assembly and annotation
DOI	10.3389/fgene.2019.01340
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31672267]; National Natural Science Foundation of China[31640074]; Jiangsu Agriculture Science and Technology Innovation Fund[CX(18) 3027]; Natural Science Foundation of Jiangsu Province[BK20171276]; Natural Science Foundation of Jiangsu Province[BK20160444]; China Postdoctoral Science Foundation[2018M642105]; National Key R&D Programof China[2018YFD0900201]; Qing Lan Project of Daizhen Zhang
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000510907600001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/165335
专题	中国科学院海洋研究所
通讯作者	Wang, Qun; Qiu, Gaofeng; Zhang, Daizhen; Li, Yongxin
作者单位	1.Yancheng Teachers Univ, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Jiangsu Key Lab Bioresources Saline Soils, Yancheng, Peoples R China 2.Northwestern Polytech Univ, Ctr Ecol & Environm Sci, Xian, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Zhejiang Ocean Univ, Natl Engn Lab Marine Germplasm Resources Explorat, Zhoushan, Peoples R China 5.Zhejiang Ocean Univ, Natl Engn Res Ctr Facilitated Marine Aquaculture, Zhoushan, Peoples R China 6.Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr & Rural Affairs, Shanghai, Peoples R China 7.China Hong Kong Ecol Consultant Co, Hong Kong, Peoples R China 8.Nanjing Normal Univ, Coll Life Sci, Nanjing, Peoples R China 9.East China Normal Univ, Sch Life Sci, Dept Biol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Tang, Boping,Wang, Zhongkai,Liu, Qiuning,et al. High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution[J]. FRONTIERS IN GENETICS,2020,10:9.
APA	Tang, Boping.,Wang, Zhongkai.,Liu, Qiuning.,Zhang, Huabin.,Jiang, Senhao.,...&Li, Yongxin.(2020).High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution.FRONTIERS IN GENETICS,10,9.
MLA	Tang, Boping,et al."High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution".FRONTIERS IN GENETICS 10(2020):9.

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