Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain

doi:10.1016/j.str.2019.11.020

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	Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain
	Bi, Yunchen 1,2,3; Zimmer, Jochen 1
	2020-02-04
发表期刊	STRUCTURE
ISSN	0969-2126
卷号	28 期号:2 页码:252-+
通讯作者	Zimmer, Jochen([email protected])
摘要	A hallmark of Gram-negative bacteria is an asymmetric outer membrane containing lipopolysaccharides (LPSs) in the extracellular leaflet. LPS molecules consist of lipid A, which is connected to the inner and outer core oligosaccharides. This LPS core structure is extended in the periplasm by the O antigen, a variable and serotype-defining polysaccharide. In the ABC transporter-dependent LPS biosynthesis pathway, the WzmWzt transporter secretes the complete O antigen across the inner membrane for ligation to the LPS core. In some O antigen transporters, the nucleotide-binding domain of Wzt is fused C-terminally to a carbohydrate-binding domain (CBD) that interacts with the O antigen chain. Here, we present the crystal structure of the Aquifex aeolicus CBD that reveals a conserved flat and a variable twisted jelly-roll surface. The CBD dimer is stabilized by mutual beta strand exchange. Microbial glycan array binding studies with the isolated CBD provide insights into its interaction with complex carbohydrates.
DOI	10.1016/j.str.2019.11.020
收录类别	SCI
语种	英语
资助项目	NIH[1R01GM129666]; Center for Functional Glycomics[R24 GM098791]; DOE Office of Science[DE-SC0012704]; National Center for Functional Glycomics[P41 GM103694]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号	WOS:000512394400013
出版者	CELL PRESS
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/165281
专题	实验海洋生物学重点实验室
通讯作者	Zimmer, Jochen
作者单位	1.Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, Charlottesville, VA 22903 USA 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Bi, Yunchen,Zimmer, Jochen. Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain[J]. STRUCTURE,2020,28(2):252-+.
APA	Bi, Yunchen,&Zimmer, Jochen.(2020).Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain.STRUCTURE,28(2),252-+.
MLA	Bi, Yunchen,et al."Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain".STRUCTURE 28.2(2020):252-+.

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Structure and Ligand（2880KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览