Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection

doi:10.1016/j.aquaculture.2012.11.014

IOCAS-IR > 实验海洋生物学重点实验室

	Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection
	Sun, Yumiao; Li, Fuhua; Xiang, Jianhai; Li, FH
	2013-02-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	376 页码:124-132
文章类型	Article
摘要	White spot syndrome virus (WSSV) is the most severe viral pathogen to the shrimp culture industry worldwide. The temporal and spatial dynamic changes of WSSV in Chinese shrimp Fenneropenaeus chinensis maintained at 22 +/- 1 degrees C during infection via injection were studied through the quantitative real-time PCR method. The pleopods were selected for virus quantification at 0, 6, 12, 18, 24, 27, 30, 33, 36, 39, 42, 45 and 48 hours post-injection (hpi). Besides the pleopod, different tissues including the hepatopancreas, nerve, heart, eyestalk, muscle, lymphoid organ, gut, stomach, gill and skin were also selected for viral load analysis at 0, 12, 27 and 30 hpi as well as the moribund stage after 40 hpi. The curve of viral load change in vivo assumed a very similar shape with the typical viral one-step growth curve. The infection process was classified into three stages: the light infection stage (0 to 18 hpi) when the viral load in the pleopod was at the level of nomore than 10(3)/ng DNA and no mortality appeared; the moderate infection stage (18 to 45 hpi), corresponding to the logarithmic phase of WSSV replication, when the viral load in the pleopod ranged from 10(3) to 10(5)/ng DNA and a low mortality rate was observed; and the heavy infection stage when WSSV propagation went into the plateau phage and massive mortality happened. A turning point from chronic infection to acute infection was suggested from 27 to 30 hpi when the viral load in most tissues increased substantially. The progress rate of WSSV in different tissues during the infection was different and the most favorite replication tissue of WSSV in early infection is the stomach, followed by the gill. The heaviest infected tissues are the skin, stomach and gill, explaining the symptoms of skin color changes, reduction of food consumption and gathering at the water surface due to dyspnea. The hepatopancreas, muscle and nerve were not the main replication sites in advanced infection. Also, the expression profiles of two anti-virus genes FcALF and FcAV were analyzed. This study might provide important data to study the virus infection mechanism. (C) 2012 Elsevier B.V. All rights reserved.; White spot syndrome virus (WSSV) is the most severe viral pathogen to the shrimp culture industry worldwide. The temporal and spatial dynamic changes of WSSV in Chinese shrimp Fenneropenaeus chinensis maintained at 22 +/- 1 degrees C during infection via injection were studied through the quantitative real-time PCR method. The pleopods were selected for virus quantification at 0, 6, 12, 18, 24, 27, 30, 33, 36, 39, 42, 45 and 48 hours post-injection (hpi). Besides the pleopod, different tissues including the hepatopancreas, nerve, heart, eyestalk, muscle, lymphoid organ, gut, stomach, gill and skin were also selected for viral load analysis at 0, 12, 27 and 30 hpi as well as the moribund stage after 40 hpi. The curve of viral load change in vivo assumed a very similar shape with the typical viral one-step growth curve. The infection process was classified into three stages: the light infection stage (0 to 18 hpi) when the viral load in the pleopod was at the level of nomore than 10(3)/ng DNA and no mortality appeared; the moderate infection stage (18 to 45 hpi), corresponding to the logarithmic phase of WSSV replication, when the viral load in the pleopod ranged from 10(3) to 10(5)/ng DNA and a low mortality rate was observed; and the heavy infection stage when WSSV propagation went into the plateau phage and massive mortality happened. A turning point from chronic infection to acute infection was suggested from 27 to 30 hpi when the viral load in most tissues increased substantially. The progress rate of WSSV in different tissues during the infection was different and the most favorite replication tissue of WSSV in early infection is the stomach, followed by the gill. The heaviest infected tissues are the skin, stomach and gill, explaining the symptoms of skin color changes, reduction of food consumption and gathering at the water surface due to dyspnea. The hepatopancreas, muscle and nerve were not the main replication sites in advanced infection. Also, the expression profiles of two anti-virus genes FcALF and FcAV were analyzed. This study might provide important data to study the virus infection mechanism. (C) 2012 Elsevier B.V. All rights reserved.
关键词	Wssv Viral Load Dynamic Changes Fenneropenaeus Chinensis
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/j.aquaculture.2012.11.014
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000313569100018
WOS关键词	WHITE-SPOT-SYNDROME ; SYNDROME VIRUS WSSV ; POLYMERASE-CHAIN-REACTION ; REAL-TIME PCR ; PENAEUS-MONODON ; ANTILIPOPOLYSACCHARIDE FACTOR ; BACULOVIRUS WSBV ; QUANTITATIVE PCR ; GENOME SEQUENCE ; GENE
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：80[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16526
专题	实验海洋生物学重点实验室
通讯作者	Li, FH
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Sun, Yumiao,Li, Fuhua,Xiang, Jianhai,et al. Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection[J]. AQUACULTURE,2013,376:124-132.
APA	Sun, Yumiao,Li, Fuhua,Xiang, Jianhai,&Li, FH.(2013).Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection.AQUACULTURE,376,124-132.
MLA	Sun, Yumiao,et al."Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection".AQUACULTURE 376(2013):124-132.

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