An opioid growth factor receptor (OGFR) for [Met(5)]-enkephalin in Chlamys farreri

doi:10.1016/j.fsi.2013.02.002

IOCAS-IR > 实验海洋生物学重点实验室

	An opioid growth factor receptor (OGFR) for [Met(5)]-enkephalin in Chlamys farreri
	Guo, Ying 1,2; Wang, Lingling 1; Zhou, Zhi 1; Wang, Mengqiang1 ; Liu, Rui 1; Wang, Leilei 1,2; Jiang, Qiufen 1,2; Song, Linsheng 1; Song, LS
	2013-05-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	34 期号:5 页码:1228-1235
文章类型	Article
摘要	Opioid growth factor receptor (OGFR) is a receptor for [Met(5)]-enkephalin and plays important roles in the regulation of cell growth and embryonic development. In the present study, a cDNA of 2381 bp for the scallop Chlamys farreri OGFR (designated as CfOGFR) was identified by rapid amplification of cDNA ends (RACE) approach and expression sequence tag (EST) analysis. The complete cDNA sequence of CfOGFR contained an open reading frame (ORF) of 1200 bp, which encoded a protein of 399 amino acids. The amino acid sequence of CfOGFR shared 33-64% similarity with other OGFRs. There was a low complexity domain and a conserved OGFR_N domain at the N-terminal of CfOGFR The mRNA transcripts of CfOGFR were constitutively expressed in the tested tissues with the highest expression level in hepatopancreas. During the early embryonic development, the mRNA transcripts of CfOGFR could be detected in different development stages, where the expression level presented a downward trend as a whole. The stimulations of LPS, Glu and poly (I:C) significantly induced the expression of CfOGFR mRNA in hemocytes (P < 0.05), while PGN stimulation exerted no influence. Co-IP and western blot results revealed that the CfOGFR in hemocytes displayed high affinity and specificity to [Met(5)]-enkephalin. Exogenous [Met(5)]-enkephalin was observed to inhibit the proliferation of HEK293T cells transfected with pcDNA3.1(+)-CfOGFR in a time and dosage dependent manner. These results collectively indicated that CfOGFR, as a homolog of OGFRs in C farreri, played an important role in cells proliferation, and might be involved in the immune response of scallops. (C) 2013 Elsevier Ltd. All rights reserved.; Opioid growth factor receptor (OGFR) is a receptor for [Met(5)]-enkephalin and plays important roles in the regulation of cell growth and embryonic development. In the present study, a cDNA of 2381 bp for the scallop Chlamys farreri OGFR (designated as CfOGFR) was identified by rapid amplification of cDNA ends (RACE) approach and expression sequence tag (EST) analysis. The complete cDNA sequence of CfOGFR contained an open reading frame (ORF) of 1200 bp, which encoded a protein of 399 amino acids. The amino acid sequence of CfOGFR shared 33-64% similarity with other OGFRs. There was a low complexity domain and a conserved OGFR_N domain at the N-terminal of CfOGFR The mRNA transcripts of CfOGFR were constitutively expressed in the tested tissues with the highest expression level in hepatopancreas. During the early embryonic development, the mRNA transcripts of CfOGFR could be detected in different development stages, where the expression level presented a downward trend as a whole. The stimulations of LPS, Glu and poly (I:C) significantly induced the expression of CfOGFR mRNA in hemocytes (P < 0.05), while PGN stimulation exerted no influence. Co-IP and western blot results revealed that the CfOGFR in hemocytes displayed high affinity and specificity to [Met(5)]-enkephalin. Exogenous [Met(5)]-enkephalin was observed to inhibit the proliferation of HEK293T cells transfected with pcDNA3.1(+)-CfOGFR in a time and dosage dependent manner. These results collectively indicated that CfOGFR, as a homolog of OGFRs in C farreri, played an important role in cells proliferation, and might be involved in the immune response of scallops. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri [Met(5)]-enkephalin Opioid Growth Factor Receptor Cell Proliferation Immune Response
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2013.02.002
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000317870600026
WOS关键词	CELL-PROLIFERATION ; GENE-EXPRESSION ; DNA-SYNTHESIS ; PHENYLALANINE-HYDROXYLASE ; IMMUNE-RESPONSE ; MYTILUS-EDULIS ; ENKEPHALIN ; HEMOCYTES ; PEPTIDES ; CANCER
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16525
专题	实验海洋生物学重点实验室
通讯作者	Song, LS
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Guo, Ying,Wang, Lingling,Zhou, Zhi,et al. An opioid growth factor receptor (OGFR) for [Met(5)]-enkephalin in Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1228-1235.
APA	Guo, Ying.,Wang, Lingling.,Zhou, Zhi.,Wang, Mengqiang.,Liu, Rui.,...&Song, LS.(2013).An opioid growth factor receptor (OGFR) for [Met(5)]-enkephalin in Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,34(5),1228-1235.
MLA	Guo, Ying,et al."An opioid growth factor receptor (OGFR) for [Met(5)]-enkephalin in Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1228-1235.

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