Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Alternative Electron Transports Participate in the Maintenance of Violaxanthin De-Epoxidase Activity of Ulva sp under Low Irradiance | |
| Xie, Xiujun1; Gu, Wenhui2,3; Gao, Shan2,3; Lu, Shan4; Li, Jian5; Pan, Guanghua1; Wang, Guangce2; Shen, Songdong6; Wang, GC | |
| 2013-11-08 | |
| 发表期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 卷号 | 8期号:11页码:e78211 |
| 文章类型 | Article |
| 摘要 | The xanthophyll cycle (Xc), which involves violaxanthin de-epoxidase (VDE) and the zeaxanthin epoxidase (ZEP), is one of the most rapid and efficient responses of plant and algae to high irradiance. High light intensity can activate VDE to convert violaxanthin (Vx) to zeaxanthin (Zx) via antheraxanthin (Ax). However, it remains unclear whether VDE remains active under low light or dark conditions when there is no significant accumulation of Ax and Zx, and if so, how the Delta pH required for activation of VDE is built. In this study, we used salicylaldoxime (SA) to inhibit ZEP activity in the intertidal macro-algae Ulva sp. (Ulvales, Chlorophyta) and then characterized VDE under low light and dark conditions with various metabolic inhibitors. With inhibition of ZEP by SA, VDE remained active under low light and dark conditions, as indicated by large accumulations of Ax and Zx at the expense of Vx. When PSII-mediated linear electron transport systems were completely inhibited by SA and DCMU, alternative electron transport systems (i.e., cyclic electron transport and chlororespiration) could maintain VDE activity. Furthermore, accumulations of Ax and Zx decreased significantly when SA, DCMU, or DBMIB together with an inhibitor of chlororespiration (i.e., propyl gallate (PG)) were applied to Ulva sp. This result suggests that chlororespiration not only participates in the build-up of the necessary DpH, but that it also possibly influences VDE activity indirectly by diminishing the oxygen level in the chloroplast.; The xanthophyll cycle (Xc), which involves violaxanthin de-epoxidase (VDE) and the zeaxanthin epoxidase (ZEP), is one of the most rapid and efficient responses of plant and algae to high irradiance. High light intensity can activate VDE to convert violaxanthin (Vx) to zeaxanthin (Zx) via antheraxanthin (Ax). However, it remains unclear whether VDE remains active under low light or dark conditions when there is no significant accumulation of Ax and Zx, and if so, how the Delta pH required for activation of VDE is built. In this study, we used salicylaldoxime (SA) to inhibit ZEP activity in the intertidal macro-algae Ulva sp. (Ulvales, Chlorophyta) and then characterized VDE under low light and dark conditions with various metabolic inhibitors. With inhibition of ZEP by SA, VDE remained active under low light and dark conditions, as indicated by large accumulations of Ax and Zx at the expense of Vx. When PSII-mediated linear electron transport systems were completely inhibited by SA and DCMU, alternative electron transport systems (i.e., cyclic electron transport and chlororespiration) could maintain VDE activity. Furthermore, accumulations of Ax and Zx decreased significantly when SA, DCMU, or DBMIB together with an inhibitor of chlororespiration (i.e., propyl gallate (PG)) were applied to Ulva sp. This result suggests that chlororespiration not only participates in the build-up of the necessary DpH, but that it also possibly influences VDE activity indirectly by diminishing the oxygen level in the chloroplast. |
| 学科领域 | Science & Technology - Other Topics |
| DOI | 10.1371/journal.pone.0078211 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000327216200014 |
| WOS关键词 | PHOTOSYSTEM-II ; XANTHOPHYLL CYCLE ; CHLOROPHYLL FLUORESCENCE ; ZEAXANTHIN-EPOXIDASE ; SPINACH-CHLOROPLASTS ; OXIDATIVE STRESS ; IN-VIVO ; ARABIDOPSIS ; CHLORORESPIRATION ; ASCORBATE |
| WOS标题词 | Science & Technology |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16523 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, GC |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Nanjing Univ, Sch Life Sci, Nanjing 210008, Jiangsu, Peoples R China 5.Catholic Univ Louvain, Earth & Life Inst, B-3000 Louvain, Belgium 6.Soochow Univ, Coll Life Sci, Suzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Xiujun,Gu, Wenhui,Gao, Shan,et al. Alternative Electron Transports Participate in the Maintenance of Violaxanthin De-Epoxidase Activity of Ulva sp under Low Irradiance[J]. PLOS ONE,2013,8(11):e78211. |
| APA | Xie, Xiujun.,Gu, Wenhui.,Gao, Shan.,Lu, Shan.,Li, Jian.,...&Wang, GC.(2013).Alternative Electron Transports Participate in the Maintenance of Violaxanthin De-Epoxidase Activity of Ulva sp under Low Irradiance.PLOS ONE,8(11),e78211. |
| MLA | Xie, Xiujun,et al."Alternative Electron Transports Participate in the Maintenance of Violaxanthin De-Epoxidase Activity of Ulva sp under Low Irradiance".PLOS ONE 8.11(2013):e78211. |
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