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| A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells | |
| Wang, Feng-xia1,2; Wu, Ning3; Wei, Jian-teng4,5; Liu, Jia3; Zhao, Jin3; Ji, Ai-guo1; Lin, Xiu-kun3,6; Lin, XK | |
| 2013-08-01 | |
| 发表期刊 | BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
 |
| ISSN | 0829-8211 |
| 卷号 | 91期号:4页码:244-251 |
| 文章类型 | Article |
| 摘要 | Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 mu g/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of beta 1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity.; Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 mu g/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of beta 1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity. |
| 关键词 | Anticancer Protein Antimetastatic Activity Eupolyphaga Sinensis Beta 1-integrin Lung Cancer A549 Cells |
| 学科领域 | Biochemistry & Molecular Biology ; Cell Biology |
| DOI | 10.1139/bcb-2013-0002 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS记录号 | WOS:000321891600008 |
| WOS关键词 | PHASE-II ; CILENGITIDE ; METASTASIS ; INTEGRINS ; ANTIBODY ; GROWTH ; VENOM |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16516 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Lin, XK |
| 作者单位 | 1.Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Shandong, Peoples R China 2.Shandong Acad Sci, Inst Biol Res, Jinan 250014, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 5.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 6.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Feng-xia,Wu, Ning,Wei, Jian-teng,et al. A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells[J]. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,2013,91(4):244-251. |
| APA | Wang, Feng-xia.,Wu, Ning.,Wei, Jian-teng.,Liu, Jia.,Zhao, Jin.,...&Lin, XK.(2013).A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells.BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,91(4),244-251. |
| MLA | Wang, Feng-xia,et al."A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells".BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE 91.4(2013):244-251. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A novel protein from（2911KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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