Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization of the Genome Feature and Toxic Capacity of a Bacillus wiedmannii Isolate From the Hydrothermal Field in Okinawa Trough | |
Zhao, Yan1,2,3 ; Chen, Chen1,2,3; Gu, Han-jie1,2,3; Zhang, Jian1,2,4; Sun, Li1,2
| |
| 2019-10-25 | |
| 发表期刊 | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
 |
| ISSN | 2235-2988 |
| 卷号 | 9页码:13 |
| 通讯作者 | Zhang, Jian([email protected]) ; Sun, Li([email protected]) |
| 摘要 | The Bacillus cereus group is frequently isolated from soil, plants, food, and other environments. In this study, we report the first isolation and characterization of a B. cereus group member, Bacillus wiedmannii SR52, from the hydrothermal field in the Iheya Ridge of Okinawa Trough. SR52 was isolated from the gills of shrimp Alvinocaris longirostris, an invertebrate species found abundantly in the ecosystems of the hydrothermal vents, and is most closely related to B. wiedmannii FSL W8-0169. SR52 is aerobic, motile, and able to form endospores. SR52 can grow in NaCl concentrations up to 9%. SR52 has a circular chromosome of 5,448,361 bp and a plasmid of 137,592 bp, encoding 5,709 and 189 genes, respectively. The chromosome contains 297 putative virulence genes, including those encoding enterotoxins and hemolysins. Fourteen rRNA operons, 107 tRNAs, and 5 sRNAs are present in the chromosome, and 7 tRNAs are present in the plasmid. SR52 possesses 13 genomic islands (GIs), all on the chromosome. Comparing to FSL W8-0169, SR52 exhibits several streaking features in its genome, notably an exceedingly large number of non-coding RNAs and GIs. In vivo studies showed that following intramuscular injection into fish, SR52 was able to disseminate in tissues and cause mortality; when inoculated into mice, SR52 induced acute mortality and disseminated transiently in tissues. In vitro studies showed that SR52 possessed hemolytic activity, and the extracellular product of SR52 exhibited a strong cytotoxic effect. These results provided the first insight into the cytotoxicity and genomic feature of B. wiedmannii from the deep-sea hydrothermal environment. |
| 关键词 | Bacillus wiedmannii deep-sea hydrothermal vent cytotoxicity virulence genome |
| DOI | 10.3389/fcimb.2019.00370 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050402]; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309]; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Immunology ; Microbiology |
| WOS类目 | Immunology ; Microbiology |
| WOS记录号 | WOS:000496429300001 |
| 出版者 | FRONTIERS MEDIA SA |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/165092 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Jian; Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Deep Sea Res Ctr, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yan,Chen, Chen,Gu, Han-jie,et al. Characterization of the Genome Feature and Toxic Capacity of a Bacillus wiedmannii Isolate From the Hydrothermal Field in Okinawa Trough[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2019,9:13. |
| APA | Zhao, Yan,Chen, Chen,Gu, Han-jie,Zhang, Jian,&Sun, Li.(2019).Characterization of the Genome Feature and Toxic Capacity of a Bacillus wiedmannii Isolate From the Hydrothermal Field in Okinawa Trough.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,9,13. |
| MLA | Zhao, Yan,et al."Characterization of the Genome Feature and Toxic Capacity of a Bacillus wiedmannii Isolate From the Hydrothermal Field in Okinawa Trough".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 9(2019):13. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Characterization of （1514KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论