Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Screening of polymorphic microsatellites and their application for Saccharina angustata and Saccharina longissima population genetic analysis | |
Zhang, Jie1,2,3 ; Wang, Xiuliang1,2,3; Yao, Jianting1,2,3; Yotsukura, Norishige4; Duan, Delin1,2,3
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| 2019-10-01 | |
| 发表期刊 | JOURNAL OF APPLIED PHYCOLOGY
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| ISSN | 0921-8971 |
| 卷号 | 31期号:5页码:3295-3301 |
| 通讯作者 | Yao, Jianting([email protected]) ; Duan, Delin([email protected]) |
| 摘要 | Saccharina angustata and S. longissima are ecologically and economically important seaweeds. So far, no microsatellite marker was available to S. angustata and S. longissima due to a lack of genomic data. Here, we developed polymorphic simple sequence repeats (SSRs) in S. longissima and S. angustata and applied these markers for further population genetic analysis. Cross-amplification tests showed that 11 SSR loci exhibited high amplification rate in S. angustata and S. longissima. Polymorphic information content values (PIC) of 11 loci showed 10 loci (PIC = 0.552-0.908), except for SJ31 (PIC = 0.482), have high polymorphism (PIC > 0.5). Polymorphism tests indicated that 10 loci have high polymorphism in three varieties of S. japonica and two related species. Genetic diversity analysis confirmed the validation of these markers in accessing the genetic diversity level of these kelps. STRUCTURE and NJ tree results indicated these 10 microsatellites are effective in evaluating the genetic relationship among different kelp populations. As results, these 10 polymorphic microsatellite markers are valid to Saccharina genetic analysis. |
| 关键词 | Saccharina angustata Saccharina longissima Phaeophyceae Simple sequence repeats Cross amplification |
| DOI | 10.1007/s10811-019-01798-6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Marine Scientific and Technological Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1]; National Natural Science Foundation of China[31772848]; National Natural Science Foundation of China[31272660]; Shandong Key Sci-Technology Research Project[2016ZDJS06B2] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS类目 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000493895800047 |
| 出版者 | SPRINGER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/165035 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Yao, Jianting; Duan, Delin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China 4.Hokkaido Univ, Field Sci Ctr Northern Biosphere, Sapporo, Hokkaido, Japan |
| 第一作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Zhang, Jie,Wang, Xiuliang,Yao, Jianting,et al. Screening of polymorphic microsatellites and their application for Saccharina angustata and Saccharina longissima population genetic analysis[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(5):3295-3301. |
| APA | Zhang, Jie,Wang, Xiuliang,Yao, Jianting,Yotsukura, Norishige,&Duan, Delin.(2019).Screening of polymorphic microsatellites and their application for Saccharina angustata and Saccharina longissima population genetic analysis.JOURNAL OF APPLIED PHYCOLOGY,31(5),3295-3301. |
| MLA | Zhang, Jie,et al."Screening of polymorphic microsatellites and their application for Saccharina angustata and Saccharina longissima population genetic analysis".JOURNAL OF APPLIED PHYCOLOGY 31.5(2019):3295-3301. |
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