Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis | |
Fang, Yan2; Yang, Hongsheng1 ; Liu, Baozhong; Zhang, Libing; Yang, HS
| |
| 2013 | |
| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
 |
| ISSN | 1532-0456 |
| 卷号 | 157期号:1页码:54-62 |
| 文章类型 | Article |
| 摘要 | The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or in combination, for 7 days. Cumulative mortality of clams and mRNA expressions of the three defense components were analyzed. The highest cumulative mortality took place in the combined treatment on day 7. The expression of the three genes was up-regulated in response to treatments compared to the control with different response times and transcriptional levels; the response to combined exposure occurred earlier than to single exposure. Among the experimental groups. MvLYZ expression and MvSOD expression peaked in the combined treatment on day 3, whereas MvMT expression peaked in heavy metals treatment on day 5. Furthermore, interactive effects of heavy metals and Vibrio on transcriptional response changed over the exposure time. Therefore, transcriptional regulation of the three genes under combined exposure was more complex than under single exposure. (C) 2012 Elsevier Inc. All rights reserved.; The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or in combination, for 7 days. Cumulative mortality of clams and mRNA expressions of the three defense components were analyzed. The highest cumulative mortality took place in the combined treatment on day 7. The expression of the three genes was up-regulated in response to treatments compared to the control with different response times and transcriptional levels; the response to combined exposure occurred earlier than to single exposure. Among the experimental groups. MvLYZ expression and MvSOD expression peaked in the combined treatment on day 3, whereas MvMT expression peaked in heavy metals treatment on day 5. Furthermore, interactive effects of heavy metals and Vibrio on transcriptional response changed over the exposure time. Therefore, transcriptional regulation of the three genes under combined exposure was more complex than under single exposure. (C) 2012 Elsevier Inc. All rights reserved. |
| 关键词 | Clam Mollusk Lysozyme Metallothionein Superoxide Dismutase Heavy Metals Bacteria Mactra Veneriformis |
| 学科领域 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| DOI | 10.1016/j.cbpc.2012.10.002 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| WOS类目 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| WOS记录号 | WOS:000312470600007 |
| WOS关键词 | OYSTER CRASSOSTREA-VIRGINICA ; PACIFIC OYSTER ; MESSENGER-RNA ; MYTILUS-GALLOPROVINCIALIS ; MEDITERRANEAN MUSSEL ; DREISSENA-POLYMORPHA ; CERASTODERMA-EDULE ; CADMIUM EXPOSURE ; GENE-EXPRESSION ; CDNA CLONING |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16502 |
| 专题 | 海洋生态与环境科学重点实验室 实验海洋生物学重点实验室 |
| 通讯作者 | Yang, HS |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 2.Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Yan,Yang, Hongsheng,Liu, Baozhong,et al. Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2013,157(1):54-62. |
| APA | Fang, Yan,Yang, Hongsheng,Liu, Baozhong,Zhang, Libing,&Yang, HS.(2013).Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,157(1),54-62. |
| MLA | Fang, Yan,et al."Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 157.1(2013):54-62. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Transcriptional resp（1319KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论