Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene.

IOCAS-IR

	Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene.
	Zhou, Li; Wang, Xueying; Liu, Qinghua; Xu, Shihong; Zhao, Haixia; Han, Mingming; Wang, Yunong; Song, Zongcheng; Li, Jun
	2019-10-01
发表期刊	Marine Biotechnology (New York Springer)
ISSN	1436-2228
卷号	21 期号:5 页码:671-682
摘要	Primordial germ cells (PGCs) as the precursors of germ cells are responsible for transmitting genetic information to the next generation. Visualization of teleost PGCs in vivo is essential to research the origination and development of germ cells and facilitate further manipulation on PGCs isolation, cryopreservation, and surrogate breeding. In this study, artificially synthesized mRNAs constructed by fusing fluorescent protein coding region to the 3' untranslated region (3'UTR) of nanos3 or vasa (mCherry-Smnanos3 3'UTR or mCherry-Smvasa 3'UTR mRNA) were injected into turbot (Scophthalmus maximus) fertilized eggs for tracing PGCs. The results demonstrated that the fluorescent PGCs differentiated from somatic cells and aligned on both sides of the trunk at the early segmentation period, then migrated and located at the dorsal part of the gut where the gonad would form. In the same way, we also found that the zebrafish (Danio rerio) vasa 3'UTR could trace turbot PGCs, while the vasa 3'UTR s of marine medaka (Oryzias melastigma) and red seabream (Pagrus major) failed, although they could label the marine medaka PGCs. In addition, through comparative analysis, we discovered that some potential sequence elements in the3 'UTRs of nanos3 and vasa, such as GCACs, 62-bp U-rich regions and nucleotide 187-218 regions might be involved in PGCs stabilization. The results of this study provided an efficient, rapid, and specific non-transgenic approach for visualizing PGCs of economical marine fish in vivo.
收录类别	SCI
语种	英语
WOS研究方向	Zoology ; Reproductive Biology ; Developmental Biology
WOS类目	ZOOLOGY ; REPRODUCTIVE BIOLOGY ; DEVELOPMENTAL BIOLOGY
WOS记录号	WOS:ZOOR15512088599
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/164881
专题	中国科学院海洋研究所
作者单位	Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao, Shandong, 266071, China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Li,Wang, Xueying,Liu, Qinghua,et al. Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene.[J]. Marine Biotechnology (New York Springer),2019,21(5):671-682.
APA	Zhou, Li.,Wang, Xueying.,Liu, Qinghua.,Xu, Shihong.,Zhao, Haixia.,...&Li, Jun.(2019).Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene..Marine Biotechnology (New York Springer),21(5),671-682.
MLA	Zhou, Li,et al."Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene.".Marine Biotechnology (New York Springer) 21.5(2019):671-682.