A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata

doi:10.1093/gigascience/giz089

	A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata
	Liu, Qin 1,2,3,4; Guo, Yunhai 1,2,3,4; Zhang, Yi 1,2,3,4; Hu, Wei 1,2,3,4,5; Li, Yuanyuan 1,2,3,4; Zhu, Dan 1,2,3,4; Zhou, Zhengbin 1,2,3,4; Wu, Jiatong 1,2,3,4; Chen, Nansheng 6,7,8; Zhou, Xiao-Nong 1,2,3,4
	2019-08-01
发表期刊	GIGASCIENCE
ISSN	2047-217X
卷号	8 期号:8 页码:8
通讯作者	Chen, Nansheng([email protected]) ; Zhou, Xiao-Nong([email protected])
摘要	Background: Triatoma rubrofasciata is a widespread pathogen vector for Chagas disease, an illness that affects approximately 7 million people worldwide. Despite its importance to human health, its evolutionary origin has not been conclusively determined. A reference genome for T. rubrofasciata is not yet available. Finding: We have sequenced the genome of a female individual with T. rubrofasciata using a single molecular DNA sequencing technology (i.e., PacBio Sequel platform) and have successfully reconstructed a whole-genome (680-Mb) assembly that covers 90% of the nuclear genome (757 Mb). Through Hi-C analysis, we have reconstructed full-length chromosomes of this female individual that has 13 unique chromosomes (2n = 24 = 22 + X1 + X2) with a contig N50 of 2.72 Mb and a scaffold N50 of 50.7 Mb. This genome has achieved a high base-level accuracy of 99.99%. This platinum-grade genome assembly has 12,691 annotated protein-coding genes. More than 95.1% of BUSCO genes were single-copy completed, indicating a high level of completeness of the genome. Conclusion: The platinum-grade genome assembly and its annotation provide valuable information for future in-depth comparative genomics studies, including sexual determination analysis in T. rubrofasciata and the pathogenesis of Chagas disease.
关键词	Triatoma rubrofasciata PacBio Sequel platform Hi-C chromosomal-level assembly comparative genomics RNA-Seq Iso-Seq
DOI	10.1093/gigascience/giz089
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2016YFC1202000]; National Science and Technology Project[2018ZX10101002]; CAS Pioneer Hundred Talents Program; Taishan Scholar Project Special Fund
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics
WOS类目	Biology ; Multidisciplinary Sciences
WOS记录号	WOS:000491875600004
出版者	OXFORD UNIV PRESS
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/164872
专题	海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng; Zhou, Xiao-Nong
作者单位	1.Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai 200025, Peoples R China 2.Minist Hlth, Key Lab Parasite & Vector Biol, Shanghai 200025, Peoples R China 3.WHO Collaborating Ctr Trop Dis, Shanghai 200025, Peoples R China 4.Chinese Ctr Trop Dis Res, Shanghai 200025, Peoples R China 5.Fudan, Sch Life Sci, Dept Microbiol & Microbial Engn, Shanghai 200025, Peoples R China 6.Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China 8.Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC, Canada
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Qin,Guo, Yunhai,Zhang, Yi,et al. A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata[J]. GIGASCIENCE,2019,8(8):8.
APA	Liu, Qin.,Guo, Yunhai.,Zhang, Yi.,Hu, Wei.,Li, Yuanyuan.,...&Zhou, Xiao-Nong.(2019).A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata.GIGASCIENCE,8(8),8.
MLA	Liu, Qin,et al."A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata".GIGASCIENCE 8.8(2019):8.

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