A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus

doi:10.1007/s00438-019-01621-4

IOCAS-IR > 实验海洋生物学重点实验室

	A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus
	Wang, Yan 1,3; Wang, Baojie 1; Shao, Xuqing 6; Liu, Mei 1; Jiang, Keyong 1; Wang, Mengqiang1,4 ; Wang, Lei 1,2,5
	2019-11-14
发表期刊	MOLECULAR GENETICS AND GENOMICS
ISSN	1617-4615
页码	13
通讯作者	Wang, Mengqiang([email protected]) ; Wang, Lei([email protected])
摘要	The red claw crayfish (Cherax quadricarinatus) is an emerging and important commercial species in several countries, and is also a potential biological model in crustacean biology. However, its molecular embryonic development mechanism remains largely unknown because of a lack of genomic resources and systematic research. A comprehensive and integrated transcriptomic analysis is necessary to reveal the cell biological function, gene expression profiles, and embryo patterning that occur during embryogenesis. In the present study, transcriptomic profiles of C. quadricarinatus embryos during three developmental stages were investigated by high-throughput Illumina sequencing technology, and the genes related to development were further analyzed. In total, 49,436 unigenes were assembled and clustered, in which 13,727 were annotated in the Nonredundant database, 5087 were classified based on Gene Ontology annotations, and 2735 were associated with 189 Kyoto Encyclopedia of Genes and Genomes pathways. Furthermore, gene expression differences among the embryos stages were analyzed, and 6658 differentially expressed genes (DEGs) were identified. In total, 3300, 5211, and 1262 DEGs were identified between the eye pigments forming stage (EP) and prepare-hatching stage (PH), EP and larvae (L), as well as PH and L; meanwhile, 1595, 2540 and 680 DEGs were annotated, respectively. The fundamental developmental genes related to apoptosis, neurogenesis, and segmentation, as well as signaling pathways related to Hedgehog, MAPK, Wnt, TGF-beta and Notch, showed higher expression during the EP stage than in other two stages, indicating that the EP stage has more active biological processes than the latter stages. This transcriptome studies gene expression at different stages of embryonic development and the datasets provide a basis for understanding crustacean developmental biology and guiding seedling production.
关键词	Cherax quadricarinatus Embryonic development Transcriptome
DOI	10.1007/s00438-019-01621-4
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[U1706209]; Marine S&T Fund of Shandong Province for National Laboratory for Marine Science and Technology[2018SDKJ0502-2]
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:000496200800001
出版者	SPRINGER HEIDELBERG
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/164866
专题	实验海洋生物学重点实验室
通讯作者	Wang, Mengqiang; Wang, Lei
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China 5.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China 6.Shandong Cigna Detect Technol Co Ltd, Qingdao 266101, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wang, Yan,Wang, Baojie,Shao, Xuqing,et al. A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus[J]. MOLECULAR GENETICS AND GENOMICS,2019:13.
APA	Wang, Yan.,Wang, Baojie.,Shao, Xuqing.,Liu, Mei.,Jiang, Keyong.,...&Wang, Lei.(2019).A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus.MOLECULAR GENETICS AND GENOMICS,13.
MLA	Wang, Yan,et al."A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus".MOLECULAR GENETICS AND GENOMICS (2019):13.

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