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| Molecular cloning and expression-profile analysis of sea cucumber DNA (Cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation | |
Zhao, Ye1,2 ; Chen, Muyan3; Su, Lin1,2; Wang, Tianming4; Liu, Shilin1; Yang, Hongsheng1; Chen, MY
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| 2013-05-01 | |
| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
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| ISSN | 1096-4959 |
| 卷号 | 165期号:1页码:26-35 |
| 文章类型 | Article |
| 摘要 | The sea cucumber Apostichopus japonicus Selenka survives high summer temperature by entering aestivation, characterized by hypometabolism and global gene silencing. We investigated the hypothesis that aestivation is associated with DNA methylation-dependent epigenetic mechanisms by cloning, sequencing and measuring the transcript abundances of two genes dnmt1 and mbd2/3, which comprise the DNA methylation system in A. japonicus Selenka. The deduced amino acid sequences and characteristic motifs of sea cucumber DNMT1 and MBD2/3 showed high homology to those of their mammalian counterparts. Quantitative real-time RT-PCR analysis showed that dnmt1 and mbd2/3 genes were similarly expressed in all four tissues examined (intestine, respiratory tree, muscle and body wall). dnmt1 expression in the intestine was up-regulated during deep aestivation (P < 0.05), while mbd2/3 was over-expressed in both the intestine and respiratory tree during the same period (P < 0.01). No differences in expression levels were observed between other tissues. The results of this study suggest that DNA methylation may be involved in transcriptional silencing, and that the intestine is the major site for epigenetic regulation during aestivation in the sea cucumber. (C) 2013 Elsevier Inc. All rights reserved.; The sea cucumber Apostichopus japonicus Selenka survives high summer temperature by entering aestivation, characterized by hypometabolism and global gene silencing. We investigated the hypothesis that aestivation is associated with DNA methylation-dependent epigenetic mechanisms by cloning, sequencing and measuring the transcript abundances of two genes dnmt1 and mbd2/3, which comprise the DNA methylation system in A. japonicus Selenka. The deduced amino acid sequences and characteristic motifs of sea cucumber DNMT1 and MBD2/3 showed high homology to those of their mammalian counterparts. Quantitative real-time RT-PCR analysis showed that dnmt1 and mbd2/3 genes were similarly expressed in all four tissues examined (intestine, respiratory tree, muscle and body wall). dnmt1 expression in the intestine was up-regulated during deep aestivation (P < 0.05), while mbd2/3 was over-expressed in both the intestine and respiratory tree during the same period (P < 0.01). No differences in expression levels were observed between other tissues. The results of this study suggest that DNA methylation may be involved in transcriptional silencing, and that the intestine is the major site for epigenetic regulation during aestivation in the sea cucumber. (C) 2013 Elsevier Inc. All rights reserved. |
| 关键词 | Apostichopus Japonicus Selenka Aestivation Dnmt1 Mbd2/3 Dna Methylation |
| 学科领域 | Biochemistry & Molecular Biology ; Zoology |
| DOI | 10.1016/j.cbpb.2013.02.009 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Zoology |
| WOS类目 | Biochemistry & Molecular Biology ; Zoology |
| WOS记录号 | WOS:000318464900004 |
| WOS关键词 | APOSTICHOPUS-JAPONICUS SELENKA ; PROTEINS ; TRANSCRIPTION ; ANIMALS ; GENOME ; REPRESSION ; DEPRESSION ; INTESTINE ; CHROMATIN ; PATTERNS |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16482 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, MY |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China 4.Zhejiang Univ, Ocean Res Ctr Zhoushan, Zhoushan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Ye,Chen, Muyan,Su, Lin,et al. Molecular cloning and expression-profile analysis of sea cucumber DNA (Cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2013,165(1):26-35. |
| APA | Zhao, Ye.,Chen, Muyan.,Su, Lin.,Wang, Tianming.,Liu, Shilin.,...&Chen, MY.(2013).Molecular cloning and expression-profile analysis of sea cucumber DNA (Cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,165(1),26-35. |
| MLA | Zhao, Ye,et al."Molecular cloning and expression-profile analysis of sea cucumber DNA (Cytosine-5)-methyltransferase 1 and methyl-CpG binding domain type 2/3 genes during aestivation".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 165.1(2013):26-35. |
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