GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS

doi:10.1111/j.1529-8817.2009.00729.x

IOCAS-IR > 实验海洋生物学重点实验室

	GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS
	Liu, Fuli 1,2; Wang, Xiuliang 1; Liu, Jidong 1; Fu, Wandong 1,2; Duan, Delin 1; Yang, Yingxia 3; Wang, XL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
	2009-10-01
发表期刊	JOURNAL OF PHYCOLOGY
ISSN	0022-3646
卷号	45 期号:5 页码:1228-1233
文章类型	Article
摘要	To establish a molecular-marker-assisted system of breeding and genetic study for Laminaria japonica Aresch., amplified fragment length polymorphism (AFLP) was used to construct a genetic linkage map of L. japonica featuring 230 progeny of F(2) cross population. Eighteen primer combinations produced 370 polymorphic loci and 215 polymorphic loci segregated in a 3:1 Mendelian segregation ratio (P <= 0.05). Of the 215 segregated loci, 142 were ordered into 27 linkage groups. The length of the linkage groups ranged from 6.7 to 90.3 centimorgans (cM) with an average length of 49.6 cM, and the total length was 1,085.8 cM, which covered 68.4% of the estimated 1,586.9 cM genome. The number of mapped markers on each linkage group ranged from 2 to 12, averaging 5.3 markers per group. The average density of the markers was 1 per 9.4 cM. Based on the marker density and the resolution of the map, the constructed linkage map can satisfy the need for quantitative trait locus (QTL) location and molecular-marker-assisted breeding for Laminaria.; To establish a molecular-marker-assisted system of breeding and genetic study for Laminaria japonica Aresch., amplified fragment length polymorphism (AFLP) was used to construct a genetic linkage map of L. japonica featuring 230 progeny of F-2 cross population. Eighteen primer combinations produced 370 polymorphic loci and 215 polymorphic loci segregated in a 3:1 Mendelian segregation ratio (P <= 0.05). Of the 215 segregated loci, 142 were ordered into 27 linkage groups. The length of the linkage groups ranged from 6.7 to 90.3 centimorgans (cM) with an average length of 49.6 cM, and the total length was 1,085.8 cM, which covered 68.4% of the estimated 1,586.9 cM genome. The number of mapped markers on each linkage group ranged from 2 to 12, averaging 5.3 markers per group. The average density of the markers was 1 per 9.4 cM. Based on the marker density and the resolution of the map, the constructed linkage map can satisfy the need for quantitative trait locus (QTL) location and molecular-marker-assisted breeding for Laminaria.
关键词	Amplified Fragment Length Polymorphism Genetic Improvement Genetic Linkage Map Laminaria Japonica Quantitative Trait Locus
学科领域	Plant Sciences ; Marine & Freshwater Biology
DOI	10.1111/j.1529-8817.2009.00729.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000270901800024
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1645
专题	实验海洋生物学重点实验室
通讯作者	Wang, XL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100059, Peoples R China 3.Ludong Univ, Yantai 264025, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Fuli,Wang, Xiuliang,Liu, Jidong,et al. GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS[J]. JOURNAL OF PHYCOLOGY,2009,45(5):1228-1233.
APA	Liu, Fuli.,Wang, Xiuliang.,Liu, Jidong.,Fu, Wandong.,Duan, Delin.,...&Wang, XL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2009).GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS.JOURNAL OF PHYCOLOGY,45(5),1228-1233.
MLA	Liu, Fuli,et al."GENETIC MAPPING OF THE LAMINARIA JAPONICA (LAMINARALES, PHAEOPHYTA) USING AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS".JOURNAL OF PHYCOLOGY 45.5(2009):1228-1233.

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