Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846)

doi:10.4194/1303-2712-v20_6_02

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846)
	Tan, Xungang1,2 ; Sui, Yulei1,2,3 ; Li, Meijie1,2,3 ; Jiao, Shuang 1,2; Wu, Zhihao 1,2; You, Feng 1,2
	2020-06-01
发表期刊	TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
ISSN	1303-2712
卷号	20 期号:6 页码:421-429
摘要	The nanos gene family plays a critical role during germline development in a wide array of organisms. The characteristics of the gene structure and function of nanos1, a nanos homolog, vary from species to species. Herein, we isolated the full-length cDNA of a nanos1 homolog in the olive flounder, Paralichthys olivaceus, and analyzed its expression pattern. The full-length cDNA sequence of the olive flounder nanos1 has 1215 base pairs (bp) and contains a 112 bp 5'-untranslated region, a 684 bp open reading frame that encodes a 228 AA peptide, and a 419 bp 3'- untranslated region. Phylogenetic analyses showed that the Nanos1 homolog of the olive flounder grouped with the nanos1 of teleost fish. We detected nanos1 mRNA in all stages of embryogenesis using RT-PCR analyses. Our whole mount in situ hybridization results showed that nands1 was expressed in the diencephalon, midbrain, hindbrain, nose, medulla oblongata, retina, abdomen, and somatic gonadal cells. Our data indicate that the expression pattern of nanos1 is not consistent from species to species, supporting that nanos1 had different functions in different organisms.
关键词	Abdomen Gonads Phylogenetic analysis Teleost fish
DOI	10.4194/1303-2712-v20_6_02
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000506048700002
出版者	CENTRAL FISHERIES RESEARCH INST
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/164425
专题	实验海洋生物学重点实验室
通讯作者	Tan, Xungang
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Tan, Xungang,Sui, Yulei,Li, Meijie,et al. Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846)[J]. TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES,2020,20(6):421-429.
APA	Tan, Xungang,Sui, Yulei,Li, Meijie,Jiao, Shuang,Wu, Zhihao,&You, Feng.(2020).Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846).TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES,20(6),421-429.
MLA	Tan, Xungang,et al."Characterization of nanos1 Homolog in the Olive Flounder, Paralichthys olivaceus (Temminck & Schlegel, 1846)".TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES 20.6(2020):421-429.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
pdf_1454.pdf（846KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览