p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis

doi:10.1016/j.fsi.2019.10.048

IOCAS-IR > 实验海洋生物学重点实验室

	p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis
	Zhang, Shujing 1,3,4; Yu, Jiajia1,4 ; Wang, Hongxia 1,2; Liu, Baozhong1,2,4 ; Yue, Xin 1,2
	2019-12-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	95 页码:456-463
通讯作者	Yue, Xin([email protected])
摘要	p38 mitogen-activated protein kinases (MAPKs) are involved in the response to various extracellular stimuli via regulating gene expression. In the present study, a p38 MAPK gene (MpP38) was identified from the clam Meretrix petechialis. The full-length cDNA of MpP38 measures 1,720 bp, consisting of a 134-bp 5'-UTR, a 1,095-bp ORF and a 491-bp 3'-UTR. Both the mRNA and protein expression levels of MpP38 increased after Vibrio challenge, implying that MpP38 is involved in clam immunity. Based on our previous study, a transcription factor activated by p38 MAPK, i.e. microphthalmia-associated transcription factor (MITF), participated in clam immunity by regulating the expression of phenoloxidase (PO). Coupled with other related reports, the mechanism underlying the involvement of MpP38 in clam immunity is most likely that pathogen stimuli induce the phosphorylation of p38 MAPK and thus activate MITF to regulate the expression of the immune-related gene PO. The results obtained in this study support this mechanism. First, we found that the MpP38 phosphorylation level increased in response to Vibrio challenge. Second, as revealed by a yeast two-hybrid assay, there was a direct interaction between MpP38 and MITF. Meanwhile, inhibiting the phosphorylation of MpP38 decreased the phosphorylation level of MpMITF, implying that MpP38 phosphorylation is required for MpMITF activation. Additionally, our results showed that there was a regulatory relationship between MpP38 phosphorylation level and PO expression level. With increased MpP38 phosphorylation level, the PO expression level was also increased after Vibrio challenge; when MpP38 phosphorylation was inhibited, the PO expression level was significantly decreased. This study describes the immune function of p38 MAPK in the clam for the first time and analyses its potential underlying mechanism, which will help to elucidate the immune mechanism in the clam M. petechialis.
关键词	p38 MAPK Vibrio challenge Immune defence MITF
DOI	10.1016/j.fsi.2019.10.048
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province (China)[2018SDKJ0502-1]###3634; Youth Foundation Project from Qingdao National Laboratory for Marine Science and Technology (China)[YQ2018N007]###3635; Agriculture Research System of China[CARS-49]###3636; National Key R&D Program of China[2019YFD0900700]###3637; National Natural Science Foundation of China[NSFC 31772845]###2133; Marine S&T Fund of Shandong Province (China)[2018SDKJ0502-1]; Youth Foundation Project from Qingdao National Laboratory for Marine Science and Technology (China)[YQ2018N007]; Agriculture Research System of China[CARS-49]; National Key R&D Program of China[2019YFD0900700]; National Natural Science Foundation of China[NSFC 31772845]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000503099200044
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/164359
专题	实验海洋生物学重点实验室
通讯作者	Yue, Xin
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Shandong, Peoples R China 3.Shandong Univ Technol, Sch Life Sci, Antiaging & Regenerat Med Res Inst, Shandong Prov Key Lab Anim Cell & Dev Biol, Zibo 255049, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhang, Shujing,Yu, Jiajia,Wang, Hongxia,et al. p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,95:456-463.
APA	Zhang, Shujing,Yu, Jiajia,Wang, Hongxia,Liu, Baozhong,&Yue, Xin.(2019).p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis.FISH & SHELLFISH IMMUNOLOGY,95,456-463.
MLA	Zhang, Shujing,et al."p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis".FISH & SHELLFISH IMMUNOLOGY 95(2019):456-463.

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