Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)

doi:10.1016/j.ygcen.2019.113291

IOCAS-IR > 实验海洋生物学重点实验室

	Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)
	Ji, Li-Qin 1,2; Rao, Ying-Zhu 1,3; Zhang, Yong 4; Chen, Rong 3; Tao, Ya-Xiong 1
	2020
发表期刊	GENERAL AND COMPARATIVE ENDOCRINOLOGY
ISSN	0016-6480
卷号	285 页码:11
通讯作者	Tao, Ya-Xiong([email protected])
摘要	Melanocortin-1 receptor (MC1R) has important roles in regulating pigmentation and inflammation. Melanocortin receptor accessory protein 2 (MRAP2) modulates trafficking, ligand binding, and signaling of mammalian melanocortin receptors. However, the effect of MRAP2 on fish MC1R has not been extensively studied. Herein, we cloned the orange-spotted grouper (Epinephelus coioides) mc1r, which had a 972 bp open reading frame encoding a putative protein of 323 amino acids. Grouper mclr was mainly expressed in the brain, skin, testis, spleen, head kidney, and kidney. EcoMC1R showed high constitutive activities in both Gs-cAMP and ERK1/2 pathways, which could be differentially modulated by grouper MRAP2 (EcoMRAP2). Three agonists, including alpha-melanocyte-stimulating hormone (MSH), beta-MSH, and ACTH, could bind to EcoMC1R and dose-dependently increase intracellular cAMP production. EcoMRAP2 had no effect on the IC50 in binding assay or EC50 in cAMP assay; however, it dose-dependently decreased the cell surface expression and maximal response to the three agonists. EcoMRAP2 increased basal ERK1/2 activation but did not alter alpha-MSH-stimulated ERK1/2 activation. This study extends the knowledge base of fish MC1R pharmacology and its regulation by MRAP2.
关键词	Epinephelus coioides Orange-spotted grouper Melanocortin-1 receptor Melanocortin receptor accessory protein 2 Tissue distribution Pharmacology
DOI	10.1016/j.ygcen.2019.113291
收录类别	SCI
语种	英语
资助项目	Science and Technology Planning Project of Guangdong Province[2014A020208136]###3547; Science and Technology Planning Project of Zhanjiang[2018A03027]###3548; Key Science Project of Lingnan Normal University[LZL1508]###3549; Guangdong Provincial Natural Science Foundation[20188030311026]###3550; Guangdong Provincial Science and Technology Program[2017B0202450001]###3551; Guangdong Provincial Science and Technology Program[2017B090904022]###3552; Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams###3553; Special Fund for Fisheries -Scientific Research of Guangdong Province[SDYY-2018-04]###3554; Science and Technology Planning Project of Guangzhou[201804020013]###3555; Independent Project of Guangdong Province Laboratory[ZJW-2019-06]###3556; Overseas Scholarship Program for Elite Young and Middle-aged Teachers of Lingnan Normal University###3557; Auburn University Animal Health and Disease Research Program of College of Veterinary Medicine at Auburn University###3558; China Scholarship Council, People's Republic of China###3559; Science and Technology Planning Project of Guangdong Province[2014A020208136]; Science and Technology Planning Project of Zhanjiang[2018A03027]; Key Science Project of Lingnan Normal University[LZL1508]; Guangdong Provincial Natural Science Foundation[20188030311026]; Guangdong Provincial Science and Technology Program[2017B0202450001]; Guangdong Provincial Science and Technology Program[2017B090904022]; Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams; Special Fund for Fisheries -Scientific Research of Guangdong Province[SDYY-2018-04]; Science and Technology Planning Project of Guangzhou[201804020013]; Independent Project of Guangdong Province Laboratory[ZJW-2019-06]; Overseas Scholarship Program for Elite Young and Middle-aged Teachers of Lingnan Normal University; Auburn University Animal Health and Disease Research Program of College of Veterinary Medicine at Auburn University; China Scholarship Council, People's Republic of China
WOS研究方向	Endocrinology & Metabolism
WOS类目	Endocrinology & Metabolism
WOS记录号	WOS:000504341800020
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/164131
专题	实验海洋生物学重点实验室
通讯作者	Tao, Ya-Xiong
作者单位	1.Auburn Univ, Coll Vet Med, Dept Anat Physiol & Pharmacol, Auburn, AL 36849 USA 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Lingnan Normal Univ, Life Sci & Technol Sch, Inst Appl Biotechnol, Zhanjiang 524048, Guangdong, Peoples R China 4.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Southern Lab Ocean Sci & Engn Zhuhai, Zhuhai 51900, Guangdong, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ji, Li-Qin,Rao, Ying-Zhu,Zhang, Yong,et al. Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2020,285:11.
APA	Ji, Li-Qin,Rao, Ying-Zhu,Zhang, Yong,Chen, Rong,&Tao, Ya-Xiong.(2020).Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides).GENERAL AND COMPARATIVE ENDOCRINOLOGY,285,11.
MLA	Ji, Li-Qin,et al."Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)".GENERAL AND COMPARATIVE ENDOCRINOLOGY 285(2020):11.

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