Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis | |
Du, Yang1,2; Fang, Han2,3 ; Shao, Xuqing5; Liu, Mei1,2; Jiang, Keyong1,2; Wang, Mengqiang1,2; Wang, Baojie1,2; Wang, Lei1,2,4
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| 2019-12-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 95页码:368-382 |
| 通讯作者 | Wang, Baojie([email protected]) ; Wang, Lei([email protected]) |
| 摘要 | Our previous work showed that using Lactobacillus pentosus HC-2 as a probiotic could improve the growth performance, immune response, gut bacterial diversity and disease resistance of Litopenaeus vannamei. However, the probiotic mechanism had not been fully characterized. In the present study, histology and proteomic analysis were performed to explore the influence of HC-2 surface protein on its probiotic effects on L. vannamei after feeding either the intact surface proteins, the probiotic treated with lithium chloride (LiCl) to remove noncovalently bound surface proteins or no probiotic for four weeks. Histological observation found that feeding with normal HC-2 obviously improved the intestinal histology and enhanced the protective effect against pathogen damage, but feeding with LiCl-treated HC-2 did not improve the intestinal environment. A total of over 2764 peptides and 1118 uniproteins were identified from the L. vannamei midgut; 211 proteins were significantly differentially expressed in the normal HC-2 group compared with the control group; 510 proteins were significantly differentially expressed in the LiCl-treated HC-2 group compared with the control group, and 458 proteins were significantly differentially expressed in the LiCl-treated HC-2 group compared with the normal HC-2 group. GO/KEGG enrichment analysis of the significantly different proteins demonstrated that feeding normal HC-2 mainly induced immune response, metabolic, cell adhesion and cell-cell signaling-related protein upregulation, which contributed to bacterial adhesion and colonization in the midgut to improve the shrimp immune system and growth, but these proteins were suppressed after the shrimp were fed bacteria deprived of surface proteins. Taken together, these results indicate that the surface proteins were indispensable for HC-2 to execute probiotic effects in the shrimp midgut. |
| 关键词 | Litopenaeus vannamei Lactobacillus pentosus HC-2 Surface proteins Proteomic analysis |
| DOI | 10.1016/j.fsi.2019.10.059 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[41906107]###3528; China Postdoctoral Science Foundation[2018M632736]###395; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH]###394; National Natural Science Foundation of China[41906107]; China Postdoctoral Science Foundation[2018M632736]; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000503099200036 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/164110 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Baojie; Wang, Lei |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China 5.Shandong Cigna Detect Technol Co Ltd, Qingdao 266237, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Du, Yang,Fang, Han,Shao, Xuqing,et al. Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,95:368-382. |
| APA | Du, Yang.,Fang, Han.,Shao, Xuqing.,Liu, Mei.,Jiang, Keyong.,...&Wang, Lei.(2019).Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis.FISH & SHELLFISH IMMUNOLOGY,95,368-382. |
| MLA | Du, Yang,et al."Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis".FISH & SHELLFISH IMMUNOLOGY 95(2019):368-382. |
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