Insulin-like growth factor-binding protein-3 plays an important role in regulating pharyngeal skeleton and inner ear formation and differentiation

doi:10.1074/jbc.M411479200

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	Insulin-like growth factor-binding protein-3 plays an important role in regulating pharyngeal skeleton and inner ear formation and differentiation
	Li, Y ; Xiang, JH; Duan, CM ; Duan, CM, Univ Michigan, Dept Mol Cellular & Dev Biol, Kraus Nat Sci Bldg, Ann Arbor, MI 48109 USA
	2005-02-04
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN	0021-9258
卷号	280 期号:5 页码:3613-3620
文章类型	Article
摘要	Insulin-like growth factor-binding protein (IGFBP)-3 is the major insulin-like growth factor (IGF) carrier protein in the bloodstream. IGFBP-3 prolongs the half-life of circulating IGFs and prevents their potential hypo-glycemic effect. IGFBP-3 is also expressed in many peripheral tissues in fetal and adult stages. In vitro, IGFBP-3 can inhibit or potentiate IGF actions and even possesses IGF-independent activities, suggesting that local IGFBP-3 may also have paracrine/autocrine function(s). The in vivo function of IGFBP-3, however, is unclear. In this study, we elucidate the developmental role of IGFBP-3 using the zebrafish model. IGFBP-3 mRNA expression is first detected in the migrating cranial neural crest cells and subsequently in pharyngeal arches in zebrafish embryos. IGFBP-3 mRNA is also persistently expressed in the developing inner ears. To determine the role of IGFBP-3 in these tissues, we ablated the IGFBP-3 gene product using morpholino-modified antisense oligonucleotides (MOs). The IGFBP-3 knocked down embryos had delayed pharyngeal skeleton morphogenesis and greatly reduced pharyngeal cartilage differentiation. Knockdown of IGFBP-3 also significantly decreased inner ear size and disrupted hair cell differentiation and semicircular canal formation. Furthermore, reintroduction of a MO-resistant form of IGFBP-3 "rescued" the MO-induced defects. These findings suggest that IGFBP-3 plays an important role in regulating pharyngeal cartilage and inner car development and growth in zebrafish.; Insulin-like growth factor-binding protein (IGFBP)-3 is the major insulin-like growth factor (IGF) carrier protein in the bloodstream. IGFBP-3 prolongs the half-life of circulating IGFs and prevents their potential hypo-glycemic effect. IGFBP-3 is also expressed in many peripheral tissues in fetal and adult stages. In vitro, IGFBP-3 can inhibit or potentiate IGF actions and even possesses IGF-independent activities, suggesting that local IGFBP-3 may also have paracrine/autocrine function(s). The in vivo function of IGFBP-3, however, is unclear. In this study, we elucidate the developmental role of IGFBP-3 using the zebrafish model. IGFBP-3 mRNA expression is first detected in the migrating cranial neural crest cells and subsequently in pharyngeal arches in zebrafish embryos. IGFBP-3 mRNA is also persistently expressed in the developing inner ears. To determine the role of IGFBP-3 in these tissues, we ablated the IGFBP-3 gene product using morpholino-modified antisense oligonucleotides (MOs). The IGFBP-3 knocked down embryos had delayed pharyngeal skeleton morphogenesis and greatly reduced pharyngeal cartilage differentiation. Knockdown of IGFBP-3 also significantly decreased inner ear size and disrupted hair cell differentiation and semicircular canal formation. Furthermore, reintroduction of a MO-resistant form of IGFBP-3 "rescued" the MO-induced defects. These findings suggest that IGFBP-3 plays an important role in regulating pharyngeal cartilage and inner car development and growth in zebrafish.
关键词	Factor Igf Binding Factor-i Messenger-rnas Developmental Expression Cellular-localization Targeted Disruption Nuclear Transport Cancer Cells Zebrafish Receptor
学科领域	Biochemistry & Molecular Biology
DOI	10.1074/jbc.M411479200
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000226983900060
引用统计	被引频次：64[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1639
专题	实验海洋生物学重点实验室
通讯作者	Duan, CM, Univ Michigan, Dept Mol Cellular & Dev Biol, Kraus Nat Sci Bldg, Ann Arbor, MI 48109 USA
作者单位	1.Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA 2.Chinese Acad Sci, Grad Sch, Beijing 10039, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Y,Xiang, JH,Duan, CM,et al. Insulin-like growth factor-binding protein-3 plays an important role in regulating pharyngeal skeleton and inner ear formation and differentiation[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2005,280(5):3613-3620.
APA	Li, Y,Xiang, JH,Duan, CM,&Duan, CM, Univ Michigan, Dept Mol Cellular & Dev Biol, Kraus Nat Sci Bldg, Ann Arbor, MI 48109 USA.(2005).Insulin-like growth factor-binding protein-3 plays an important role in regulating pharyngeal skeleton and inner ear formation and differentiation.JOURNAL OF BIOLOGICAL CHEMISTRY,280(5),3613-3620.
MLA	Li, Y,et al."Insulin-like growth factor-binding protein-3 plays an important role in regulating pharyngeal skeleton and inner ear formation and differentiation".JOURNAL OF BIOLOGICAL CHEMISTRY 280.5(2005):3613-3620.

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