Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei

doi:10.1016/j.chemosphere.2019.124759

IOCAS-IR > 实验海洋生物学重点实验室

	Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei
	Liu, Fei 1,2; Li, Shihao 1,2,3; Yu, Yang1,2,3 ; Sun, Mingzhe1,2,4 ; Xiang, Jianhai1,2 ; Li, Fuhua 1,2,3
	2020
发表期刊	CHEMOSPHERE
ISSN	0045-6535
卷号	239 页码:12
通讯作者	Li, Fuhua([email protected])
摘要	Ammonia is an important environmental stress factor in aquaculture. Long-term ammonia stress could affect the normal growth, and also increase the risk for the occurrence of various diseases. In order to learn the mechanism that ammonia caused the outbreak of the shrimp disease, transcriptomics and metabolomics approaches were used to analyze the differential expressions of the genes in hemocytes and different metabolites in the serum of the Pacific white shrimp Litopenaeus vannamei under ammonia exposure. Transcriptional analysis showed that 17 cell apoptosis related genes, seven phagocytosis related genes, 10 immunity related genes and seven cell cycle and lipid metabolism related genes showed differential expressions after ammonia exposure. Metabolomics analysis on the serum showed that 25 differential metabolites were identified in positive and negative ion patterns. They are involved in purine metabolism, amino acids metabolism and lipid metabolism. Injection of two up-regulated metabolites triethanolamine and oxypurinol to normal shrimp could induce apoptosis in normal shrimp. The total hemocytes counts in shrimp showed a significant decrease and the apoptotic cell ratio increased significantly under ammonia exposure. These results suggested that ammonia exposure increased the apoptosis of hemocytes, which affected the immunity of shrimp, and thus caused susceptibility to pathogenic infection. These data will help us understand the mechanism of ammonia stress leading to the immunity decline of shrimp. (C) 2019 Elsevier Ltd. All rights reserved.
关键词	Ammonia stress Metabolome Transcriptome Hemocytes Litopenaeus vannamei
DOI	10.1016/j.chemosphere.2019.124759
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31830100]###95; National Natural Science Foundation of China[41776158]###147; National Natural Science Foundation of China[31772880]###146; China Agriculture Research System-48[CARS-48]###1408; National Natural Science Foundation of China[31830100]; National Natural Science Foundation of China[41776158]; National Natural Science Foundation of China[31772880]; China Agriculture Research system-48[CARS-48]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000498305500068
出版者	PERGAMON-ELSEVIER SCIENCE LTD
引用统计	被引频次：74[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163865
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Fei,Li, Shihao,Yu, Yang,et al. Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei[J]. CHEMOSPHERE,2020,239:12.
APA	Liu, Fei,Li, Shihao,Yu, Yang,Sun, Mingzhe,Xiang, Jianhai,&Li, Fuhua.(2020).Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei.CHEMOSPHERE,239,12.
MLA	Liu, Fei,et al."Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei".CHEMOSPHERE 239(2020):12.

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