Molecular responses of an estuarine oyster to multiple metal contamination in Southern China revealed by RNA-seq

doi:10.1016/j.scitotenv.2019.134648

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular responses of an estuarine oyster to multiple metal contamination in Southern China revealed by RNA-seq
	Li, Yunlong 1,2; Zhang, Xinhui 3; Meng, Jie4 ; Chen, Jieming 3; You, Xinxin 3; Shi, Qiong 3; Wang, Wen-Xiong 1,2
	2020-01-20
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	701 页码:11
通讯作者	Wang, Wen-Xiong([email protected])
摘要	The estuarine oysters Crassostrea hongkongensis hyper-accumulate many metals and survive under high levels of metal exposure. In the present study, three natural populations of oysters with various levels of accumulated metals (mainly Cu and Zn) were collected from Southern China. The morphological characteristics and metal concentrations revealed their phenotypic differentiation. Further transcripts sequences acquired from their gill tissues were analyzed and 44,801 genes (with effective reads) were obtained via de novo assembly. The principal component analysis (PCA) revealed that the gene expression patterns also displayed differentiation among the three populations. A total of 3,199 differentially expressed genes (DEGs) was identified in the contaminated oysters as compared to the 'clean' oysters, which were used to explain the molecular mechanisms of metal accumulation and toxicity. GO and KEGG enrichment analysis revealed that energy production and cytoskeleton metabolism-related genes were particularly enriched in the contaminated sites during chronic metal exposure. Besides, increasing expressions of Zn/Cu transporters and metallothionein may explain their high accumulation in contaminated populations. We showed that oysters with less metal accumulation tended to cope with metal stress actively, but severe contamination destroyed part of the normal function. Our study analyzed the gene expression patterns of C. hongkongensis in Southern China and demonstrated the phenotypic differentiation of oysters under chronical metal exposure in the field. (C) 2019 Elsevier B.V. All rights reserved.
关键词	Oysters Metal contamination Field population Estuary
DOI	10.1016/j.scitotenv.2019.134648
收录类别	SCI
语种	英语
资助项目	Research Grant Council of Hong Kong SAR[16140616]###3437; Research Grant Council of Hong Kong SAR[16140616]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000498801400044
出版者	ELSEVIER
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163811
专题	实验海洋生物学重点实验室
通讯作者	Wang, Wen-Xiong
作者单位	1.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Kowloon, Clear Water Bay, Hong Kong, Peoples R China 2.Hong Kong Univ Sci & Technol, Hong Kong Branch, Kowloon, Southern Marine Sci & Engn Guangdong Lab Guangzho, Clear Water Bay, Hong Kong, Peoples R China 3.BGI, Shenzhen Key Lab Marine Genom, Guangdong Prov Key Lab Mol Breeding Marine Econ A, BGI Marine,BGI Acad Marine Sci, Shenzhen 518083, Guangdong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Li, Yunlong,Zhang, Xinhui,Meng, Jie,et al. Molecular responses of an estuarine oyster to multiple metal contamination in Southern China revealed by RNA-seq[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,701:11.
APA	Li, Yunlong.,Zhang, Xinhui.,Meng, Jie.,Chen, Jieming.,You, Xinxin.,...&Wang, Wen-Xiong.(2020).Molecular responses of an estuarine oyster to multiple metal contamination in Southern China revealed by RNA-seq.SCIENCE OF THE TOTAL ENVIRONMENT,701,11.
MLA	Li, Yunlong,et al."Molecular responses of an estuarine oyster to multiple metal contamination in Southern China revealed by RNA-seq".SCIENCE OF THE TOTAL ENVIRONMENT 701(2020):11.

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