Effects of the probiotic Bacillus amyloliquefaciens on the growth, immunity, and disease resistance of Haliotis discus hannai

doi:10.1016/j.fsi.2019.08.067

IOCAS-IR > 实验海洋生物学重点实验室

	Effects of the probiotic Bacillus amyloliquefaciens on the growth, immunity, and disease resistance of Haliotis discus hannai
	Gao Xiaolong1,2 ; Ke Caihuan 1; Zhang Mo 2; Li Xian 2; Wu Fucun 2; Liu Ying3
	2019-11-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	94 页码:617-627
通讯作者	Liu Ying([email protected])
摘要	The effects of a diet containing the probiotic Bacillus amyloliquefaciens on the survival and growth of Haliotis discus hannai were evaluated by measuring growth and hematological parameters and the expression levels of nonspecific immune genes. In addition, the abalones response to Vibrio parahaemolyticus infection was assessed. H. discus hannai (shell length: 29.35 +/- 1.81 mm, body weight: 4.28 +/- 0.23 g) were exposed to an 8-week culture experiment in indoor aquariums and a 2-week V. parahaemolyticus artificial infection experiment. In each experiment, the control group (C) was fed daily with the basal feed; the experimental groups were fed daily with the experimental feed, prepared by spraying B. amyloliquefaciens onto the basal feed at final concentrations of 10(3) (group A1), 10(5) (A2), and 10(7) (A3) cfu/g. The survival rate, body weight specific growth rate, and food conversion efficiency in A2 and A3 were significantly higher than those in A1 and C (P < 0.05). The total number of blood lymphocytes, the O2- and NO levels produced from respiratory burst, the activities of acid phosphatase, superoxide dismutase, and catalase, and the expression levels of catalase and thiol peroxidase in A2 were not significantly different from those in A3, but these factors were significantly higher in A2 compared to Al and C (P < 0.05). The total antioxidant capacity and expression levels of glutathione S-transferase in Al, A2 and A3 were significantly higher than those in C (P < 0.05). At day 9 after infection with V. parahaemolyticus, all abalone in C were dead; at the end of the experiment, the cumulative mortality of abalone in A2 was significantly lower than that in any other group (P < 0.05). Thus, the experimental feed containing 10(3) cfu/g B. amyloliquefaciens not only facilitated the food intake and growth of abalone, but also effectively enhanced their non-specific immunity and resistance to V. parahaemolyticus infection. In this regard, B. amyloliquefaciens may be a useful probiotic strain for abalone aquaculture.
关键词	Bacillus amyloliquefaciens Haliotis discus hannai Growth Immunity Disease resistance
DOI	10.1016/j.fsi.2019.08.067
收录类别	SCI
语种	英语
资助项目	earmarked fund for the Modern Agro-industry Technology Research System[CARS-49]###3169; China Postdoctoral Science Foundation[2019M650153]###3307; National Natural Science Foundation of China of China[31672673]###3428; State Key Laboratory of Marine Environmental Science at Xiamen University###3309; earmarked fund for the Modern Agro-industry Technology Research System[CARS-49]; China Postdoctoral Science Foundation[2019M650153]; National Natural Science Foundation of China of China[31672673]; State Key Laboratory of Marine Environmental Science at Xiamen University
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000496892400065
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163796
专题	实验海洋生物学重点实验室
通讯作者	Liu Ying
作者单位	1.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Fujian, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Dalian Ocean Univ, Dalian 116023, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gao Xiaolong,Ke Caihuan,Zhang Mo,et al. Effects of the probiotic Bacillus amyloliquefaciens on the growth, immunity, and disease resistance of Haliotis discus hannai[J]. FISH & SHELLFISH IMMUNOLOGY,2019,94:617-627.
APA	Gao Xiaolong,Ke Caihuan,Zhang Mo,Li Xian,Wu Fucun,&Liu Ying.(2019).Effects of the probiotic Bacillus amyloliquefaciens on the growth, immunity, and disease resistance of Haliotis discus hannai.FISH & SHELLFISH IMMUNOLOGY,94,617-627.
MLA	Gao Xiaolong,et al."Effects of the probiotic Bacillus amyloliquefaciens on the growth, immunity, and disease resistance of Haliotis discus hannai".FISH & SHELLFISH IMMUNOLOGY 94(2019):617-627.

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