Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection

doi:10.1016/j.bios.2012.07.078

	Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection
	Qi, Peng1,2 ; Wan, Yi 1,2; Zhang, Dun 1; Zhang, D
	2013-01-15
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	39 期号:1 页码:282-288
文章类型	Article
摘要	This work presents the synthesis of bacteria-mediated bioimprinted films for selective bacterial detection. Marine pathogen sulfate-reducing bacteria (SRB) were chosen as the template bacteria. Chitosan (CS) doped with reduced graphene sheets (RGSs) was electrodeposited on an indium tin oxide electrode, and the resulting RGSs-CS hybrid film served as a platform for bacterial attachment. The electrodeposition conditions were optimized to obtain RGSs-CS hybrid films with excellent electrochemical performance. A layer of nonconductive CS film was deposited to embed the pathogen, and acetone was used to wash away the bacterial templates. Electrochemical impedance spectroscopy was performed to characterize the stepwise modification process and monitor the SRB population. Faradic impedance measurements revealed that the charge transfer resistance (R-ct) increased with increased SRB concentration. A linear relationship between Delta R-ct and the logarithm of SRB concentration was obtained within the concentration range of 1.0 x 10(4) cfu mL(-1) to 1.0 x 10(8) cfu mL(-1). The impedimetric sensor showed good selectivity towards SRB based on size and shape. Hence, selectivity for bacterial detection can be improved if the bioimprinting technique is combined with other bio-recognition elements. (C) 2012 Elsevier B.V. All rights reserved.; This work presents the synthesis of bacteria-mediated bioimprinted films for selective bacterial detection. Marine pathogen sulfate-reducing bacteria (SRB) were chosen as the template bacteria. Chitosan (CS) doped with reduced graphene sheets (RGSs) was electrodeposited on an indium tin oxide electrode, and the resulting RGSs-CS hybrid film served as a platform for bacterial attachment. The electrodeposition conditions were optimized to obtain RGSs-CS hybrid films with excellent electrochemical performance. A layer of nonconductive CS film was deposited to embed the pathogen, and acetone was used to wash away the bacterial templates. Electrochemical impedance spectroscopy was performed to characterize the stepwise modification process and monitor the SRB population. Faradic impedance measurements revealed that the charge transfer resistance (R-ct) increased with increased SRB concentration. A linear relationship between Delta R-ct and the logarithm of SRB concentration was obtained within the concentration range of 1.0 x 10(4) cfu mL(-1) to 1.0 x 10(8) cfu mL(-1). The impedimetric sensor showed good selectivity towards SRB based on size and shape. Hence, selectivity for bacterial detection can be improved if the bioimprinting technique is combined with other bio-recognition elements. (C) 2012 Elsevier B.V. All rights reserved.
关键词	Sulfate-reducing Bacteria Graphene Electrochemical Impedance Spectroscopy Bioimprinted Film Electrodeposition
学科领域	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
DOI	10.1016/j.bios.2012.07.078
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号	WOS:000309486300046
WOS关键词	SULFATE-REDUCING BACTERIA ; MOLECULARLY IMPRINTED POLYMERS ; GRAPHENE SHEETS ; NANOPARTICLES ; SURFACE ; HYBRIDIZATION ; FLUORESCENCE ; VANCOMYCIN ; PATHOGENS ; ELECTRODE
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
引用统计	被引频次：81[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16372
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zhang, D
作者单位	1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Corros Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Qi, Peng,Wan, Yi,Zhang, Dun,et al. Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection[J]. BIOSENSORS & BIOELECTRONICS,2013,39(1):282-288.
APA	Qi, Peng,Wan, Yi,Zhang, Dun,&Zhang, D.(2013).Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection.BIOSENSORS & BIOELECTRONICS,39(1),282-288.
MLA	Qi, Peng,et al."Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection".BIOSENSORS & BIOELECTRONICS 39.1(2013):282-288.

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